Whitcomb D C, O'Dorisio T M, Cataland S, Shetzline M A, Nishikawara M T
Am J Physiol. 1985 Dec;249(6 Pt 1):E561-7. doi: 10.1152/ajpendo.1985.249.6.E561.
An in vivo radioreceptor assay for polypeptide hormones has been developed and applied to the identification of tissue insulin receptors. The theoretical basis for this assay is presented elsewhere in this issue. 125I-insulin and 131I-albumin were infused into male rats with increasing amounts of unlabeled insulin. Plasma samples were taken at 1-min intervals until the animals were killed at 5 min. Tissue samples were excised and weighed and the activity due to each isotope counted. By comparing the differential distribution of the labeled tracers and applying the results to a compartment model, the specific, displaceable binding of insulin to tissue receptors could be demonstrated. Binding was detected in the liver, muscle, fat, adrenal glands, pancreas, small intestines, and spleen.
已开发出一种用于多肽激素的体内放射受体测定法，并将其应用于组织胰岛素受体的鉴定。该测定法的理论基础在本期其他地方介绍。将125I-胰岛素和131I-白蛋白与越来越多的未标记胰岛素一起注入雄性大鼠体内。每隔1分钟采集血浆样本，直到5分钟后处死动物。切除组织样本并称重，对每种同位素的活性进行计数。通过比较标记示踪剂的差异分布并将结果应用于房室模型，可以证明胰岛素与组织受体的特异性、可置换结合。在肝脏、肌肉、脂肪、肾上腺、胰腺、小肠和脾脏中检测到结合。
