Whitcomb D C, O'Dorisio T M, Cataland S, Nishikawara M T
Am J Physiol. 1985 Dec;249(6 Pt 1):E555-60. doi: 10.1152/ajpendo.1985.249.6.E555.
To date the in vivo identification and quantitation of specific hormone receptors has been difficult, time consuming, and lacking in sensitivity. We present the theory underlying a new in vivo radioreceptor assay for polypeptide hormones based on receptor theory derived from in vitro investigations and in vivo kinetic and autoradiographic studies. The assay was developed from a tissue model and a theory of hormone distribution. Measuring the labeled hormone distributed between the plasma and interstitial space in the presence or absence of excess unlabeled hormone permits the accurate determination of hormone specifically bound to receptors. This approach eliminates the problem of nonspecific binding due to free tracer, hormone degradation products, or labeled non-hormone molecules. A receptor compartment and specific binding of hormone are calculated from only four measured parameters: total tissue labeled hormone, tissue albumin, plasma labeled hormone, and plasma albumin. The method is applicable to most tissues and hormones under a variety of conditions and permits simultaneous comparison of multiple tissues in the same animal under identical conditions.
迄今为止，特定激素受体的体内鉴定和定量一直困难重重、耗时费力且缺乏灵敏度。我们提出了一种基于体外研究以及体内动力学和放射自显影研究得出的受体理论，用于多肽激素体内放射受体测定的理论基础。该测定方法是从组织模型和激素分布理论发展而来的。在存在或不存在过量未标记激素的情况下，测量分布于血浆和细胞间质中的标记激素，可准确测定与受体特异性结合的激素。这种方法消除了因游离示踪剂、激素降解产物或标记的非激素分子导致的非特异性结合问题。仅通过四个测量参数：组织总标记激素、组织白蛋白、血浆标记激素和血浆白蛋白，即可计算出受体区室和激素的特异性结合。该方法适用于多种条件下的大多数组织和激素，并允许在相同条件下同时比较同一动物的多个组织。
