Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Medicine, Jilin Agricultural University, Changchun, Jilin 130118, China.
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
Poult Sci. 2024 Oct;103(10):104086. doi: 10.1016/j.psj.2024.104086. Epub 2024 Jul 10.
Probiotics are increasingly recognized for their capacity to combat pathogenic bacteria. In this study, we isolated a strain of Ligilactobacillus salivarius XP132 from the gut microbiota of healthy chickens. This strain exhibited resistance to low pH and bile salts, auto-aggregation capabilities, and the ability to co-aggregate with pathogenic Salmonella. The in vitro antibacterial activity of Ligilactobacillus salivarius XP132 was tested using an Oxford cup antibacterial test, and the results showed that Ligilactobacillus salivarius XP132 exhibited broad-spectrum antibacterial activity, with especially strong antibacterial activity against Salmonella. In animal experiments with white feather broilers and specific-pathogens-free (SPF) chickens, we orally administered 1 × 10 CFU XP132 live bacteria per chicken per day, and detected the content of Salmonella in the liver, spleen, intestinal contents, and eggs of the chickens by RT-qPCR. Oral administration of Lactobacillus salivarius XP132 group significantly reduced the levels of Salmonella in chicken liver, spleen, intestinal contents and eggs, and the oral administration of Ligilactobacillus salivarius XP132 significantly inhibited the horizontal and vertical transmission of Salmonella in SPF chickens and white-feathered broilers. After oral administration of XP132, the production of chicken serum anti-infective cytokine IFN-γ was also significantly up-regulated, thereby enhancing the host's ability to resist infection. In addition, the production of various serum inflammatory cytokines, including IL-1β, IL-6, IL-8, and TNF-α, was down-regulated, leading to significant amelioration of the inflammatory response induced by S. Pullorum in chickens. These findings suggest that Ligilactobacillus salivarius XP132 possesses potent antibacterial and immunomodulatory properties that effectively prevent both horizontal and vertical transmission of Salmonella Pullorum, highlighting its potential as a valuable tool for the prevention and control of Salmonella disease.
益生菌因其对抗致病菌的能力而越来越受到重视。在本研究中，我们从健康鸡的肠道微生物群中分离出一株唾液乳杆菌 XP132。该菌株表现出对低 pH 值和胆汁盐的抗性、自动聚集能力以及与致病性沙门氏菌的共聚集能力。采用牛津杯抑菌试验检测唾液乳杆菌 XP132 的体外抑菌活性，结果表明唾液乳杆菌 XP132 具有广谱抑菌活性，对沙门氏菌的抑菌活性尤其强。在白羽肉鸡和 SPF 鸡的动物实验中，我们每天每只鸡口服 1×10 CFU XP132 活菌，通过 RT-qPCR 检测鸡肝、脾、肠内容物和鸡蛋中沙门氏菌的含量。口服唾液乳杆菌 XP132 组可显著降低鸡肝、脾、肠内容物和鸡蛋中沙门氏菌的含量，口服唾液乳杆菌 XP132 可显著抑制 SPF 鸡和白羽肉鸡中沙门氏菌的水平和垂直传播。口服 XP132 后，鸡血清抗感染细胞因子 IFN-γ 的产生也显著上调，从而增强了宿主的抗感染能力。此外，血清中各种炎症细胞因子，包括 IL-1β、IL-6、IL-8 和 TNF-α 的产生也下调，导致鸡感染鸡白痢沙门氏菌引起的炎症反应得到显著改善。这些发现表明唾液乳杆菌 XP132 具有强大的抑菌和免疫调节特性，可有效预防沙门氏菌 Pullorum 的水平和垂直传播，突出了其作为预防和控制沙门氏菌病的有价值工具的潜力。
