Alani Zaid Khalid, Kawan May Hameed
College of Health Medical Techniques, Al-Bayan University, Baghdad, Iraq.
College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
J Adv Vet Anim Res. 2024 Jun 9;11(2):392-397. doi: 10.5455/javar.2024.k788. eCollection 2024 Jun.
This study aimed to detect in cats by microscopic and molecular analysis using PCR, sequencing, and phylogenetic analysis.
Randomly selected 200 cat feces samples were taken from various private veterinarian clinics in Baghdad. To identify eggs of by the flotation method, DNA from 100 cat feces was extracted, and one pair of ITS2 region-specific primers was used for polymerase chain reaction, followed by sequencing.
infection rate was found to be 23 out of 100 fecal samples using PCR. Ten DNA product sequence data studies showed 98%-100% similarity to the 5.8S ribosomal RNA gene sequences found in the Gene Bank. The study incidence showed that the overall infection rate by microscopic examination was 23%, with no significant difference between stray cats (27%), and domestic cats (19%). After studying the effect of several epidemiological parameters on the infection rate, it was found that the infection rates of stray and domestic cats were higher in kittens under six months of age, at 46.1% and 27%, respectively, whereas rates were lower for the adult than six months was 11.5% of domestic cats and 14.7% of stray cats. The percentage of stray and domestic male cats that were registered was 35.5%, whereas the female cats registered were 20.6% and 17.5%, respectively.
Cats are significant clinical reservoirs for zoonotic parasites. In Iraq, Baghdad has a high incidence of detections. Compared to conventional methods, PCR is thought to be a more sensitive, accurate diagnostic procedure that confirms the species' identity.
本研究旨在通过聚合酶链反应（PCR）、测序和系统发育分析，利用显微镜和分子分析方法在猫身上进行检测。
从巴格达的各个私人兽医诊所随机选取200份猫粪便样本。采用漂浮法鉴定虫卵，从100份猫粪便中提取DNA，使用一对ITS2区域特异性引物进行聚合酶链反应，随后进行测序。
使用PCR方法在100份粪便样本中发现感染率为23%。十项DNA产物序列数据研究表明，与基因库中发现的5.8S核糖体RNA基因序列相似度为98%-100%。研究发病率显示，显微镜检查的总体感染率为23%，流浪猫（27%）和家猫（19%）之间无显著差异。在研究了几个流行病学参数对感染率的影响后发现，六个月龄以下的小猫中，流浪猫和家猫的感染率较高，分别为46.1%和27%，而成猫的感染率低于六个月龄的家猫为11.5%，流浪猫为14.7%。登记的流浪雄猫和家猫的百分比为35.5%，而登记的雌猫分别为20.6%和17.5%。
猫是重要的人畜共患寄生虫临床宿主。在伊拉克，巴格达的检测发病率较高。与传统方法相比，PCR被认为是一种更敏感、准确的诊断程序，可确认物种身份。
