Laila Sri Rahmatul, Sukmawinata Eddy, Kencana Putri Falsa Martiana, Akbar Ilham, Zahra Luthfiyyah Fitri, Agungpriyono Srihadi, Cahyaningsih Umi, Wresdiyati Tutik
Division of Anatomy, Histology, and Embryology, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, Indonesia.
Research Centre for Veterinary Science, Research Organization for Health, National Research and Innovation Agency, Bogor, Indonesia.
J Adv Vet Anim Res. 2024 Jun 5;11(2):275-283. doi: 10.5455/javar.2024.k773. eCollection 2024 Jun.
We aimed to evaluate the potential of (AD), a combination of Indonesian traditional fermented buffalo milk () and black glutinous rice flakes () as an anti-hypercholesterolemic agent and to prevent liver-cell degeneration using a rat model.
A mixture of black glutinous rice powder (0.3 gm/gm feed) and fermented buffalo milk (3.74/200 gm BW) was prepared to obtain AD. Fifteen adult male Sprague Dawley rats were divided into three groups of five animals each: (A) negative control group (distilled water; 5 weeks), (B) hypercholesterolemia group (1% cholesterol per feed; 5 weeks), and (C) preventive AD group (1% cholesterol and AD; 5 weeks). The blood lipid profiles were measured at weeks 2, 4, and 5. The liver enzyme activity, cholesterol level, and histology were observed at the end of week 5.
AD administration simultaneously with cholesterol in Group C significantly prevented an increase in total plasma cholesterol and low-density lipoprotein levels compared to Group B. Alanine transaminase and aspartate transaminase were maintained at normal levels in Group C. Furthermore, the levels of liver cholesterol and liver cell degeneration in Group C were also maintained because of AD administration compared to that in Group B.
This study demonstrated that AD has the potential to be developed as a functional food for hypercholesterolemia prevention.
我们旨在评估印尼传统发酵水牛奶（DB）和黑糯米片（BB）的组合产品（AD）作为抗高胆固醇血症药物以及使用大鼠模型预防肝细胞变性的潜力。
制备黑糯米粉（0.3克/克饲料）和发酵水牛奶（3.74/200克体重）的混合物以获得AD。将15只成年雄性斯普拉格 - 道利大鼠分为三组，每组五只动物：（A）阴性对照组（蒸馏水；5周），（B）高胆固醇血症组（每饲料含1%胆固醇；5周），以及（C）预防性AD组（1%胆固醇和AD；5周）。在第2、4和5周测量血脂谱。在第5周结束时观察肝酶活性、胆固醇水平和组织学情况。
与B组相比，C组中AD与胆固醇同时给药显著预防了总血浆胆固醇和低密度脂蛋白水平的升高。C组中的丙氨酸转氨酶和天冬氨酸转氨酶维持在正常水平。此外，与B组相比，由于给予AD，C组中的肝脏胆固醇水平和肝细胞变性情况也得到了维持。
本研究表明，AD有潜力被开发为预防高胆固醇血症的功能性食品。
