Husna Asmaul, Kallol Md Arefin, Ferdous Farhana Binte, Lima Khudaza Akter, Tumpa Zannatul Haque, Khan Mohammad Ferdousur Rahman, Rahman Marzia
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh.
J Adv Vet Anim Res. 2024 Jun 19;11(2):455-462. doi: 10.5455/javar.2024.k795. eCollection 2024 Jun.
This study focused on the antibiogram profiling of and coagulase-negative spp (CoNS) and the detection of and genes from bovine raw milk samples.
Bovine milk samples were collected from dairy farms, and spp. were isolated and identified via conventional and molecular screening. Disk diffusion test (DDT) was implemented to determine the resistance pattern. Biofilm and β-lactamase-producing spp. were identified via amplification of the and genes. Methicillin-resistant and CoNS were identified by DDT and PCR of the gene.
From 63 samples, 35 were confirmed as spp., of which 16 (25.39%) isolates were coagulase-positive, while 19 (30.16%) were negative. PCR confirmed that 50% (8/16) of and 36.84% (7/19) of CoNS possessed the gene. All isolates were found resistant to penicillin-G (P) both phenotypically and genotypically. The isolates were also resistant to erythromycin (ERY) and oxytetracycline (TET). While CoNS showed high to reduced resistance against P, TET, ERY, and azithromycin, no isolates were resistant to sulfamethoxazole, while 10.53% of CoNS isolates were. All and CoNS isolates were susceptible to vancomycin and gentamicin. MR was exhibited by 37.5% of and 42.10% of CoNS isolates. Moreover, and CoNS had 56.25% and 52.63% multidrug-resistant (MDR) isolates, respectively.
The present study revealed the presence of a biofilm-producing, MDR staphylococcal strain in milk that might endanger consumers. Routine surveillance and monitoring, along with antimicrobial resistance learning, can reduce risks.
本研究聚焦于金黄色葡萄球菌和凝固酶阴性葡萄球菌属（CoNS）的抗菌谱分析以及从牛生鲜乳样本中检测mecA和blaZ基因。
从奶牛场采集牛乳样本，通过常规和分子筛查分离并鉴定葡萄球菌属。采用纸片扩散法（DDT）确定耐药模式。通过扩增icaA和blaZ基因鉴定生物被膜和产β-内酰胺酶的葡萄球菌属。通过DDT和mecA基因的PCR鉴定耐甲氧西林金黄色葡萄球菌和CoNS。
在63个样本中，35个被确认为葡萄球菌属，其中16株（25.39%）金黄色葡萄球菌分离株凝固酶阳性，而19株（30.16%）为阴性。PCR证实50%（8/16）的金黄色葡萄球菌和36.84%（7/19）的CoNS携带mecA基因。所有金黄色葡萄球菌分离株在表型和基因型上均对青霉素G（P）耐药。这些分离株还对红霉素（ERY）和土霉素（TET）耐药。虽然CoNS对P、TET、ERY和阿奇霉素显示出高至降低的耐药性，但没有金黄色葡萄球菌分离株对磺胺甲恶唑耐药，而10.53%的CoNS分离株耐药。所有金黄色葡萄球菌和CoNS分离株对万古霉素和庆大霉素敏感。37.5%的金黄色葡萄球菌和42.10%的CoNS分离株表现出耐甲氧西林特性。此外，金黄色葡萄球菌和CoNS分别有56.25%和52.63%的多重耐药（MDR）分离株。
本研究揭示了牛奶中存在产生物被膜的多重耐药葡萄球菌菌株，这可能危及消费者。常规监测以及对抗菌药物耐药性的了解可降低风险。
