Mithun Ferdous Hasan, Bhuiyan Md Eftakhar Jahan, Hossain Md Golzar, Debnath Chirojit, Nazir K H M Nazmul Hussain, Akter Sharmin
Department of Physiology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
J Adv Vet Anim Res. 2024 Jun 22;11(2):503-515. doi: 10.5455/javar.2024.k800. eCollection 2024 Jun.
The experiment aimed to determine the effects of lipopolysaccharide (LPS), polymyxin B, and honey on survival rates, hematological parameters, liver and kidney biomarkers, blood glucose levels, serum insulin levels, and histopathology of the liver, kidney, lungs, brain, and pancreas in LPS-challenged mice.
50 male Swiss Albino mice (), aged 3 weeks, were randomly assigned into 5 groups (10 mice per group): Control group (A), LPS (2 mg/kg bwt/day IP in NS) treated group (B), polymyxin B (1.2 mg/kg bwt/day IM) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (C), honey (10 gm/kg bwt/day PO) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (D), both polymyxin B (1.2 mg/kg bwt/day IM) and honey (10 gm/kg bwt/day PO) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (E). The LPS was administered intraperitoneally (IP) at 80 µg/mice/day, diluting in normal saline. After 16 weeks, the mice were sacrificed, and blood samples and organs (liver, kidney, lung, brain, and pancreas) were collected for laboratory tests.
The results revealed that in LPS-treated mice, the mortality rate was the highest, and hemato-biochemical parameters were altered. Histopathological examination in the group treated with LPS showed disarrangement of hepatocytes, cellular infiltrations in the glomerulus, alveolar congestion in the lungs, several nerve fiber degenerations in the brain, and degenerative changes in pancreatic islets. The mortality rate and hemato-biochemical and histopathological changes were restored by the combined treatment of polymyxin B and honey.
LPS has detrimental effects on survival rate and hemato-biochemistry, which are lessened by taking honey and polymyxin B supplements.
本实验旨在确定脂多糖（LPS）、多粘菌素B和蜂蜜对脂多糖攻击小鼠的存活率、血液学参数、肝肾生物标志物、血糖水平、血清胰岛素水平以及肝脏、肾脏、肺、脑和胰腺组织病理学的影响。
50只3周龄雄性瑞士白化小鼠被随机分为5组（每组10只）：对照组（A）、LPS（以2mg/kg体重/天腹腔注射于生理盐水中）处理组（B）、多粘菌素B（以1.2mg/kg体重/天肌肉注射）预处理加LPS（以2mg/kg体重/天腹腔注射于生理盐水中）处理组（C）、蜂蜜（以10g/kg体重/天口服）预处理加LPS（以2mg/kg体重/天腹腔注射于生理盐水中）处理组（D）、多粘菌素B（以1.2mg/kg体重/天肌肉注射）和蜂蜜（以10g/kg体重/天口服）联合预处理加LPS（以2mg/kg体重/天腹腔注射于生理盐水中）处理组（E）。LPS以80μg/小鼠/天腹腔注射，用生理盐水稀释。16周后，处死小鼠，采集血样和器官（肝脏、肾脏、肺、脑和胰腺）进行实验室检测。
结果显示，LPS处理的小鼠死亡率最高，血液生化参数发生改变。LPS处理组的组织病理学检查显示肝细胞排列紊乱、肾小球细胞浸润、肺肺泡充血、脑内多条神经纤维变性以及胰岛退行性改变。多粘菌素B和蜂蜜联合治疗可恢复死亡率以及血液生化和组织病理学变化。
LPS对存活率和血液生化有有害影响，服用蜂蜜和多粘菌素B补充剂可减轻这些影响。
