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（麦卢卡）氯仿提取物对乳腺癌和肝癌细胞系的生长抑制作用。

Growth inhibitory effect of (manuka) chloroform extract on breast and liver cancer cell lines.

作者信息

Al-Zharani Mohammed

机构信息

Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia.

出版信息

J Adv Vet Anim Res. 2024 Jun 4;11(2):237-246. doi: 10.5455/javar.2024.k769. eCollection 2024 Jun.

DOI:10.5455/javar.2024.k769
PMID:39101096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11296193/
Abstract

OBJECTIVE

Research has demonstrated that possesses various therapeutic benefits. This study set out to determine whether or not extracts had any effect on the ability of HepG2 and MCF-7 breast cancer cells to survive.

MATERIALS AND METHODS

The antiproliferative activity of extracts was explored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. The most active fraction was selected to investigate its effects on apoptosis induction using flow cytometry and quantitative real-time polymerase chain reaction. The constituents of this fraction were characterized using GC-MS analysis.

RESULTS

Research demonstrated that the chloroform fraction of (LSCF) significantly impacted the HepG2 and MCF-7 cancer cell lines. Treatment with LSCF led to a notable rise in both early and late apoptotic cells. Furthermore, there was an upregulation in the mRNA levels of P53, Bax, and caspases, while the expression of Bcl-2 mRNA saw a decrease. The analysis of LSCF revealed the primary components to be cis-calamenene, beta-eudesmol, cyclododecane, and alpha-muurolene.

CONCLUSION

The study showed the promising antiproliferative activity of , suggesting its potential application for cancer treatment.

摘要

目的

研究表明[具体物质未提及]具有多种治疗益处。本研究旨在确定[具体物质未提及]提取物对HepG2和MCF - 7乳腺癌细胞存活能力是否有任何影响。

材料与方法

使用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐和乳酸脱氢酶测定法探索[具体物质未提及]提取物的抗增殖活性。选择活性最高的馏分，使用流式细胞术和定量实时聚合酶链反应研究其对凋亡诱导的影响。使用气相色谱 - 质谱分析对该馏分的成分进行表征。

结果

研究表明[具体物质未提及]的氯仿馏分（LSCF）对HepepGepG2和MCF - 7癌细胞系有显著影响。用LSCF处理导致早期和晚期凋亡细胞均显著增加。此外，P53、Bax和半胱天冬酶的mRNA水平上调，而Bcl - 2 mRNA的表达下降。LSCF分析显示主要成分是顺式菖蒲烯、β - 桉叶醇、环十二烷和α - 榄香烯。

结论

该研究表明[具体物质未提及]具有有前景的抗增殖活性，表明其在癌症治疗中的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/9ebe91c41a5d/JAVAR-11-237-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/295dc0a9212d/JAVAR-11-237-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/77dcb9114ad0/JAVAR-11-237-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/f0d44b878b13/JAVAR-11-237-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/a3a113121974/JAVAR-11-237-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/a12b6919efec/JAVAR-11-237-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/f6341159f670/JAVAR-11-237-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/9ebe91c41a5d/JAVAR-11-237-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/295dc0a9212d/JAVAR-11-237-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/77dcb9114ad0/JAVAR-11-237-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/f0d44b878b13/JAVAR-11-237-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/a3a113121974/JAVAR-11-237-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/a12b6919efec/JAVAR-11-237-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/f6341159f670/JAVAR-11-237-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da82/11296193/9ebe91c41a5d/JAVAR-11-237-g007.jpg

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