State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Ministry of Agriculture and Rural Affairs Key Laboratory of Post-Harvest Handling of Fruits, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
Int J Biol Macromol. 2024 Oct;278(Pt 1):134463. doi: 10.1016/j.ijbiomac.2024.134463. Epub 2024 Aug 3.
Previous studies have shown that modified citrus pectin (MCP) is an anti-tumor material of food grade. In this study, two enzymatically modified Ougan (Citrus Suavissima Hort. ex Tanaka) peel pectins (EMP1 and EMP2, the ones extracted by alkali and enzymatic methods) were used to investigate their differential effects on viability and physiology of Hela cells. The results showed that EMP1 and EMP2 had 88.00 % and 81.01 % galacturonic acid, 21.31 % and 20.25 % esterification degree, 10,417 g/mol and 6416 g/mol molecular weight (Mw), 82.86 % and 50.62 % RG-I, and 8.91 % and 15.70 % HG, respectively. EMP2 had higher intensities of absorption peaks than EMP1. They were irregularly shaped, with more holes on EMP1 but more wrinkles on EMP2. Both could inhibit the growth, proliferation, migration, and invasion of HeLa cells in a concentration-dependent manner, with better efficiency in EMP2. Meanwhile, EMP2 was more efficient than EMP1 in blocking the cell cycle in S phase, resulting in apoptosis. In conclusion, the variations caused by extraction resulted in differences in anti-tumor activity of MCP and EMP2 with lower Mw and higher HG exhibited better anti-tumor effects. This study would provide an experimental basis and reference for the research and development of anti-tumor supplements from citrus pectin.
先前的研究表明，改性柑橘果胶（MCP）是一种食品级的抗肿瘤物质。在本研究中，使用两种酶法改性的瓯柑（Citrus Suavissima Hort. ex Tanaka）果皮果胶（EMP1 和 EMP2，分别通过碱法和酶法提取）来研究它们对 Hela 细胞活力和生理的差异影响。结果表明，EMP1 和 EMP2 的半乳糖醛酸含量分别为 88.00%和 81.01%，酯化度分别为 21.31%和 20.25%，分子量（Mw）分别为 10417g/mol 和 6416g/mol，RG-I 分别为 82.86%和 50.62%，HG 分别为 8.91%和 15.70%。EMP2 的吸收峰强度高于 EMP1。它们的形状不规则，EMP1 上的孔较多，而 EMP2 上的褶皱较多。两者均可浓度依赖性地抑制 HeLa 细胞的生长、增殖、迁移和侵袭，EMP2 的效率更高。同时，EMP2 比 EMP1 更有效地阻断细胞周期在 S 期，导致细胞凋亡。综上所述，提取过程中产生的变化导致 MCP 和 EMP2 的抗肿瘤活性存在差异，Mw 较低且 HG 较高的 EMP2 表现出更好的抗肿瘤效果。本研究为柑橘果胶抗肿瘤保健品的研究与开发提供了实验依据和参考。
