Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Departments of Oncology and Pathology, School of Medicine, Wayne State University, and Karmanos Cancer Institute, Detroit, MI, USA; Department of Maxillofacial Surgery, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
Carbohydr Polym. 2019 May 1;211:141-151. doi: 10.1016/j.carbpol.2019.02.010. Epub 2019 Feb 4.
While chemically and thermally modified citrus pectin (MCP) has already been studied for health benefits, it is unknown how size-fractionated oligo- and polysaccharides differentially affect cancer cell behavior. We produced thermally MCP and fractionated it by molecular size to evaluate the effect these polymers have on cancer cells. MCP30/10 (between 30 and 10 kDa) had more esterified homogalacturonans (HG) and fewer rhamnogalacturonans (RG-I) than MCP and MCP30 (higher than 30 kDa), while MCP10/3 (between 10 and 3 kDa) showed higher amounts of type I arabinogalactans (AGI) and lower amounts of RG-I. MCP3 (smaller than 3 kDa) presented less esterified HG and the lowest amount of AGI and RG-I. Our data indicate that the enrichment of de-esterified HG oligomers and the AGI and RG-I depletions in MCP3, or the increase of AGI and loss of RGI in MCP30/10, enhance the anticancer behaviors by inhibiting migration, aggregation, and proliferation of cancer cells.
虽然化学和热改性柑橘果胶（MCP）已被研究用于健康益处，但尚不清楚大小分级的低聚糖和多糖如何对癌细胞行为产生不同影响。我们生产了热 MCP，并根据分子大小对其进行了分级，以评估这些聚合物对癌细胞的影响。MCP30/10（30 至 10 kDa 之间）比 MCP 和 MCP30（高于 30 kDa）具有更多的酯化同质半乳糖醛酸聚糖（HG）和更少的鼠李半乳糖醛酸聚糖（RG-I），而 MCP10/3（10 至 3 kDa 之间）则表现出更高的 I 型阿拉伯半乳聚糖（AGI）和更低的 RG-I。MCP3（小于 3 kDa）表现出较少的酯化 HG 和最低量的 AGI 和 RG-I。我们的数据表明，MCP3 中去酯化 HG 低聚物的富集以及 AGI 和 RG-I 的减少，或 MCP30/10 中 AGI 的增加和 RG-I 的损失，通过抑制癌细胞的迁移、聚集和增殖来增强抗癌行为。
