Zhu Xiaoyan, Luo Fenglei, Zheng Yixiong, Zhang Jiukai, Huang Jianzhen, Sun Chongde, Li Xian, Chen Kunsong
Laboratory of Fruit Quality Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Int J Mol Sci. 2013 Apr 24;14(5):8684-97. doi: 10.3390/ijms14058684.
Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit.
枳属苷是一种具有多种生物活性的苦味黄酮苷。枳属苷是从瓯柑果实（Citrus reticulate cv. Suavissima）的四种不同组织（外果皮、白皮层、囊衣和汁胞）中分离得到的。瓯柑果实白皮层中枳属苷的含量最高（1.37 mg/g干重）。采用高速逆流色谱（HSCCC）结合D101树脂色谱法从瓯柑果实白皮层中分离纯化枳属苷。经过这两步纯化后，枳属苷的纯度从0.14%提高到了96.56%。通过HPLC-PDA和LC-MS对纯化后的枳属苷的化学结构进行了鉴定。枳属苷对人胃癌细胞SGC-7901的生长具有显著的体外抑制作用，且呈剂量依赖性。因此，瓯柑果实中的枳属苷可能对预防胃癌有益。本文展示的纯化方法将有助于进一步研究枳属苷活性的药理机制，以及指导瓯柑果实的消费。
