Department of Orthopaedic Trauma, Center for Orthopaedic Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China; Academy of Orthopedics · Guangdong Province, Guangzhou, China.
Department of Infection, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Int J Biol Macromol. 2024 Oct;277(Pt 4):134472. doi: 10.1016/j.ijbiomac.2024.134472. Epub 2024 Aug 3.
Chronic inflammation and infection often lead to delayed healing in skin wounds of patients with diabetes, presenting a significant challenge in clinical wound repair. In an effort to tackle this issue, we explored the utilization of the natural compounds Rhein and chitosan in the creation of a crosslinked in situ gel. Developed as Rhein-chitosan in situ hydrogel (CS-Rh gel), this formulation has the ability to gel at body temperature, making it suitable for irregular wounds of varying shapes. Our experimental investigations have demonstrated its excellent biocompatibility, controlled release of Rhein, biodegradability, anti-inflammatory properties, antibacterial effect, as well as its ability to enhance keratinocyte proliferation and migration. Furthermore, in vivo studies have confirmed that CS-Rh gel can effectively mitigate tissue inflammation, promote collagen deposition, and significantly accelerate wound healing in diabetic mice within a short timeframe of two weeks. Consequently, this innovative approach holds promise as a viable therapeutic strategy for supporting the healing of diabetic wounds in a clinical setting.
慢性炎症和感染常导致糖尿病患者皮肤伤口愈合延迟，这给临床伤口修复带来了重大挑战。为了解决这个问题，我们研究了将天然化合物 Rhein 和壳聚糖应用于交联原位凝胶的方法。我们开发了 Rhein-壳聚糖原位水凝胶（CS-Rh 凝胶），这种配方能够在体温下凝胶化，适用于各种形状的不规则伤口。实验研究表明，CS-Rh 凝胶具有良好的生物相容性、Rhein 的控制释放、生物降解性、抗炎特性、抗菌作用以及促进角质细胞增殖和迁移的能力。此外，体内研究证实 CS-Rh 凝胶可有效减轻组织炎症、促进胶原沉积，并在短短两周内显著加速糖尿病小鼠的伤口愈合。因此，这种创新方法有望成为一种可行的治疗策略，用于支持临床环境中糖尿病伤口的愈合。
