CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Environ Pollut. 2024 Nov 1;360:124675. doi: 10.1016/j.envpol.2024.124675. Epub 2024 Aug 3.
Nowadays, traditional single-omics study is not enough to explain the causality between molecular alterations and toxicity endpoints for environmental pollutants. With the development of high-throughput sequencing technology and high-resolution mass spectrometry technology, the integrative analysis of multi-omics has become an efficient strategy to understand holistic biological mechanisms and to uncover the regulation network in specific biological processes. This review summarized sample preparation methods, integration analysis tools and the application of multi-omics integration analyses in environmental toxicology field. Currently, omics methods have been widely applied being as the sensitivity of early biological response, especially for low-dose and long-term exposure to environmental pollutants. Integrative omics can reveal the overall changes of genes, proteins, and/or metabolites in the cells, tissues or organisms, which provide new insights into revealing the overall toxicity effects, screening the toxic targets, and exploring the underlying molecular mechanism of pollutants.
如今，传统的单一组学研究不足以解释环境污染物的分子变化与毒性终点之间的因果关系。随着高通量测序技术和高分辨率质谱技术的发展，多组学的综合分析已成为理解整体生物学机制和揭示特定生物学过程调控网络的有效策略。本文综述了多组学整合分析在环境毒理学领域中的样品制备方法、整合分析工具及应用。目前，组学方法已广泛应用于作为早期生物反应的敏感性研究，特别是对于低剂量和长期暴露于环境污染物的情况。整合组学可以揭示细胞、组织或生物体中基因、蛋白质和/或代谢物的整体变化，为揭示整体毒性效应、筛选毒性靶标以及探索污染物的潜在分子机制提供了新的视角。
