Lin Hui, Gao Yun-Fei, Wei Chao, Wang Meng-Lei, Ma Xu-Hui
Department of Dentistry, Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. Shanghai 200062, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2024 Jun;33(3):255-259.
To investigate the effect of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice.
Six-week-old female non-pregnant wild-type C57BL/6J mice were randomly divided into sham operation group, ovariectomy(OVX) group and OVX+Sophora japonica extract group. Ovaries of the mice in the OVX group and the OVX+Sophora japonica extract group were removed, and the mice in the OVX+Sophora japonica extract group were treated by Sophora japonica extract at a dose of 150 mg/kg, three times a week for 4 weeks; while mice of the other two groups were given an equal volume of normal saline at the same time. Body weight was measured 3 times a week, and the micro-parameters of alveolar bone were detected by Micro-CT after 4 weeks. The data were analyzed by GraphPad Prism 9 software.
Compared with the sham-operated group, the trabecular bone parameters of the alveolar bone in the OVX group were significantly decreased 1 month after operation (P＜0.05). One month after intervention with Sophora japonica extract, alveolar bone mineral density (BMD), trabecular number (Tb.N) and trabecular separation(Tb.Sp) in OVX mice was significantly rescued, with no significant difference compared to the sham surgery group(P＞0.05); but bone volume fraction(BV/TV) and trabecular thickness (Tb.Th) had not completely recovered to the levels of the sham-operated group(P＜0.05).
Sophora japonica extract can effectively increase the alveolar bone mass reduced by estrogen deficiency and may be used as one of the potential drugs for the treatment of menopausal alveolar bone osteoporosis.
探讨槐米提取物对去卵巢骨质疏松小鼠牙槽骨质量的影响。
将6周龄雌性非孕野生型C57BL/6J小鼠随机分为假手术组、去卵巢（OVX）组和OVX+槐米提取物组。OVX组和OVX+槐米提取物组小鼠切除卵巢，OVX+槐米提取物组小鼠给予150mg/kg剂量的槐米提取物治疗，每周3次，共4周；而其他两组小鼠同时给予等体积的生理盐水。每周测量3次体重，4周后通过Micro-CT检测牙槽骨的微观参数。数据采用GraphPad Prism 9软件进行分析。
与假手术组相比，OVX组术后1个月牙槽骨的小梁骨参数显著降低（P＜0.05）。槐米提取物干预1个月后，OVX小鼠的牙槽骨矿物质密度（BMD）、小梁数量（Tb.N）和小梁间距（Tb.Sp）得到显著改善，与假手术组相比无显著差异（P＞0.05）；但骨体积分数（BV/TV）和小梁厚度（Tb.Th）未完全恢复到假手术组水平（P＜0.05）。
槐米提取物可有效增加因雌激素缺乏而降低的牙槽骨质量，可能作为治疗绝经后牙槽骨骨质疏松的潜在药物之一。
