Department of Prosthodontics, Semmelweis University, Szentkirályi u. 47, Budapest, 1088, Hungary.
Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Ankara University, Emniyet Mah.İncitaş sokak.Sabancı Kız yurdu karşısı, 06500, Ankara, Turkey.
BMC Oral Health. 2021 Dec 7;21(1):627. doi: 10.1186/s12903-021-01980-8.
This study aimed to investigate the effect of ovariectomy and vitamin D3 on bone microstructure; this effect was examined in three regions of interest at one femoral and two mandibular sampling sites bone in an ovariectomized mouse model.
Thirty-six week-old female mice were randomly divided into three groups: 10 subjects were given oral cholecalciferol (vitamin D3) daily for 6 weeks after undergoing bilateral ovariectomy (D3 group), while 10 ovariectomized subjects (OVX) and 10 subjects who underwent a sham operation (SHAM) received peanut oil daily during the investigation. After extermination, the left hemimandible and femur were removed and scanned by micro-CT. The bone micromorphology parameters were analyzed and the BMD was calculated.
The bone volume fraction (BV/TV) was significantly lower in the trabecular bone of the mandibular condyle in the OVX group than in the SHAM and D3 groups. Also there was a significant difference between the SHAM and D3 groups. The specific bone surface (BS/BV) was significantly higher in the OVX and D3 groups than in the SHAM group. Trabecular thickness (Tb.Th) was significantly higher in the SHAM group, and the trabecular bone pattern factor (Tb.Pf) was significantly higher in the OVX group than in the other two groups. Bone mineral density (BMD) of the femur and the mandible was significantly lower in the OVX group than in the SHAM and D3 groups.
Our results show that ovariectomy causes a significantly weaker bone microstructure in the mandibular condyle, where the protective effect of vitamin D3 resulted in a partial resorption.
本研究旨在探讨去卵巢和维生素 D3 对骨微观结构的影响；在去卵巢小鼠模型中，在股骨和两个下颌骨采样部位的三个感兴趣区域检查了这种影响。
36 周龄雌性小鼠随机分为三组：10 只每日给予口服胆钙化醇（维生素 D3），6 周后行双侧卵巢切除术（D3 组），10 只卵巢切除术（OVX）和 10 只假手术（SHAM）接受每日花生油。处死时，取下左侧下颌骨和股骨并进行微 CT 扫描。分析骨微形态参数并计算 BMD。
OVX 组下颌骨髁突的骨小梁体积分数（BV/TV）明显低于 SHAM 和 D3 组。SHAM 和 D3 组之间也存在显著差异。OVX 和 D3 组的特定骨表面（BS/BV）明显高于 SHAM 组。SHAM 组的骨小梁厚度（Tb.Th）明显较高，OVX 组的骨小梁模式因子（Tb.Pf）明显高于其他两组。OVX 组股骨和下颌骨的骨密度（BMD）明显低于 SHAM 和 D3 组。
我们的结果表明，卵巢切除导致下颌骨髁突的骨微观结构明显减弱，而维生素 D3 的保护作用导致部分吸收。
