Piotrovskiĭ V K, Veĭko N N, Rumiantsev D O, Zhirkov Iu A, El'man A R
Farmakol Toksikol. 1985 Nov-Dec;48(6):62-7.
High performance liquid chromatography has acquired great importance recently for an analysis of the drugs in biological fluids of the body. Phase inversion sorbents are particularly widely used today. However, the use of ion-exchange sorbents is fairly promising for an analysis of the drugs capable of ionizing in an aqueous solution. The authors illustrate the use of the latter ones for determination in the blood, urine and saliva of man of a number of the cardiologic drugs (etmozine, nonachlazine, verapamil, prazosin, propranolol, nadolol). Ion-exchange sorbents make it possible to attain better results than inverse phase ones, since they retain the drugs selectively and do not retain the endogenous substances of lipid nature. As regards verapamil, prazosin and propranolol, the unchanged drug and polar metabolites could be determined jointly, with such a determination being not feasible with the use of phase inversion sorbents. Separation of the diastereoisomers of nadolol was achieved in the blood and urine of patients who received the drug.
高效液相色谱法近来在分析人体生物流体中的药物方面变得极为重要。相转化吸附剂如今应用尤为广泛。然而，离子交换吸附剂对于分析能够在水溶液中电离的药物颇具前景。作者阐述了使用后者在人体血液、尿液和唾液中测定多种心脏病药物（乙吗噻嗪、诺氯嗪、维拉帕米、哌唑嗪、普萘洛尔、纳多洛尔）的情况。离子交换吸附剂能够比反相吸附剂取得更好的结果，因为它们能选择性地保留药物，而不保留脂类性质的内源性物质。对于维拉帕米、哌唑嗪和普萘洛尔，可联合测定未变化的药物和极性代谢物，而使用相转化吸附剂则无法进行这样的测定。在服用该药物的患者的血液和尿液中实现了纳多洛尔非对映异构体的分离。
