Xu Jiaxin, Gao Yu, Ding Yuting, Feng Yunpeng, Chen Jie, Zhang Shenshen, Song Xiaoyu, Qiao Shifeng
The Second Ward of Colorectal Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Computer Teaching and Research Section, Jinzhou Medical University, Jinzhou, Liaoning, China.
Front Oncol. 2024 Jul 22;14:1373820. doi: 10.3389/fonc.2024.1373820. eCollection 2024.
To explore the mechanism by which Tregs promote the progression of colorectal cancer by inducing tumor-associated macrophages to polarize into M2 type via ICOS.
Postoperative pathological tissues and clinical pathological data of 268 colorectal cancer patients who underwent initial surgery were collected. Immunohistochemistry (IHC) was used to detect the expression levels of ICOS, CD163 (a marker for M2 macrophages), and Foxp3 (a marker for Tregs) in cancerous, adjacent non-tumorous, and normal tissues. The relationship of ICOS, M2 macrophages, and Tregs in CRC with clinical pathological characteristics and pre-surgical tumor markers (such as CEA and CA199) was explored.
The expression levels of M2 macrophages and Tregs increased with tumor progression, while ICOS expression showed a decreasing trend. Compared to adjacent and normal tissues, the expression levels of ICOS, M2 macrophages, and Tregs were higher in CRC tissues. The expression levels of M2 macrophages and Tregs were significantly positively correlated with tumor markers, while ICOS expression was significantly negatively correlated.
Tumor-associated m2 macrophages induced by Tregs and ICOS participate in the dynamic balance of the colorectal cancer tumor microenvironment, and their interaction affects colorectal carcinogenesis and progression. High levels of ICOS are associated with better long-term survival rates.
探讨调节性T细胞（Tregs）通过诱导肿瘤相关巨噬细胞经由可诱导共刺激分子（ICOS）极化为M2型从而促进结直肠癌进展的机制。
收集268例接受初次手术的结直肠癌患者的术后病理组织及临床病理资料。采用免疫组织化学（IHC）检测癌组织、癌旁非肿瘤组织及正常组织中ICOS、CD163（M2巨噬细胞标志物）和Foxp3（Tregs标志物）的表达水平。探讨结直肠癌中ICOS、M2巨噬细胞和Tregs与临床病理特征及术前肿瘤标志物（如癌胚抗原和糖类抗原199）的关系。
M2巨噬细胞和Tregs的表达水平随肿瘤进展而升高，而ICOS表达呈下降趋势。与癌旁组织和正常组织相比，结直肠癌组织中ICOS、M2巨噬细胞和Tregs的表达水平更高。M2巨噬细胞和Tregs的表达水平与肿瘤标志物呈显著正相关，而ICOS表达呈显著负相关。
Tregs和ICOS诱导的肿瘤相关M2巨噬细胞参与了结直肠癌肿瘤微环境的动态平衡，它们之间的相互作用影响结直肠癌的发生和进展。高水平的ICOS与更好的长期生存率相关。
