Liu Wenliang, Li Gongying, Zhu Mengya, Yang Lin
Department of Psychological, Huai'an No. 3 People's Hospital, Huaian, People's Republic of China.
Psychol Res Behav Manag. 2024 Aug 1;17:2875-2883. doi: 10.2147/PRBM.S462835. eCollection 2024.
To investigate the association between single nucleotide polymorphisms (SNPs) of tryptophan hydroxylase 2 (TPH2) (rs11178997, rs11178998, and rs120074175) and negative life events in adolescent depression with Non-suicidal self-injury (NSSI).
Genomic DNA was extracted from 197 adolescents with depression (participants group, including NSSI group and non-NSSI group), as well as from 100 healthy controls (control group), in northern China. PCR technology was utilized to amplify DNA fragments and detect genotypes in both groups. The Adolescent Life Event Scale (ASLEC) was employed to conduct a questionnaire survey among the participants and control groups. Differences in allele and genotype frequency distribution between the two groups were analyzed using the X^2 test, while generalized multifactor dimensionality reduction (GMDR) was used to analyze gene-environment interactions.
Significant differences were observed in ASLEC scores between the control group and both the NSSI group and non-NSSI group (P<0.05). Additionally, significant differences were found in the interpersonal relationship factor and punishment factor between the NSSI group and non-NSSI group (P < 0.05). Moreover, a significant difference was identified in SNP genotype of rs11178997 between the depression group (NSSI group + non-NSSI group) and control group (P<0.05). GMDR analysis revealed an interaction among rs11178997, rs11178998, and ASLEC.
Adolescents with depression, particularly females, may exhibit a tendency to employ NSSI as an emotional coping mechanism when confronted with greater family and interpersonal challenges. The AT genotype of TPH2 gene locus rs11178997 is more prevalent among adolescents with depression. Furthermore, the occurrence of NSSI may be associated with an interaction involving polymorphic sites rs11178997 and rs11178998 along with life events.
探讨色氨酸羟化酶2（TPH2）的单核苷酸多态性（SNP）（rs11178997、rs11178998和rs120074175）与伴有非自杀性自伤（NSSI）的青少年抑郁症患者负性生活事件之间的关联。
在中国北方，从197名抑郁症青少年（参与者组，包括NSSI组和非NSSI组）以及100名健康对照者（对照组）中提取基因组DNA。利用聚合酶链反应（PCR）技术扩增两组的DNA片段并检测基因型。采用青少年生活事件量表（ASLEC）对参与者组和对照组进行问卷调查。使用卡方检验分析两组之间等位基因和基因型频率分布的差异，同时采用广义多因素降维法（GMDR）分析基因 - 环境相互作用。
对照组与NSSI组和非NSSI组的ASLEC得分均存在显著差异（P<0.05）。此外，NSSI组与非NSSI组在人际关系因子和惩罚因子方面也存在显著差异（P<0.05）。而且，抑郁症组（NSSI组 + 非NSSI组）与对照组之间rs11178997的SNP基因型存在显著差异（P<0.05）。GMDR分析显示rs11178997、rs11178998和ASLEC之间存在相互作用。
患有抑郁症的青少年，尤其是女性，在面对更大的家庭和人际挑战时，可能会表现出采用NSSI作为情绪应对机制的倾向。TPH2基因位点rs11178997的AT基因型在抑郁症青少年中更为普遍。此外，NSSI的发生可能与rs11178997和rs111789,98多态性位点以及生活事件之间的相互作用有关。
