Kost J, Horbett T A, Ratner B D, Singh M
J Biomed Mater Res. 1985 Nov-Dec;19(9):1117-33. doi: 10.1002/jbm.820190920.
The development of membranes that swell in response to glucose is reported. The membranes may prove to be useful in glucose monitoring or glucose-dependent insulin delivery. The polymers were synthesized by the radiation-induced polymerization of frozen solutions containing hydroxyethylmethacrylate, N,N-dimethylaminoethyl methacrylate, tetraethylene glycol dimethacrylate, ethylene glycol, water, and glucose oxidase. The polymers were hydrogels, with water contents in the range of 60-90%, depending on the pH or glucose concentration. Changes in swelling and permeability of the hydrogel were caused by exposure to glucose solutions. The gluconic acid formed by the glucose oxidase catalyzed oxidation of glucose in the membrane lowered the pH of the system and thus caused the changes in the membrane. The retention of enzyme activity by the membranes in vitro and in vivo is also reported. The large differences in properties among membranes made with different chemical formulations suggest that glucose-sensitive membranes with performance characteristics needed for an artificial pancreas may be an achievable goal.
据报道，已开发出对葡萄糖有响应而膨胀的膜。这些膜可能在葡萄糖监测或葡萄糖依赖型胰岛素递送方面有用。这些聚合物是通过对含有甲基丙烯酸羟乙酯、甲基丙烯酸N,N-二甲基氨基乙酯、四甘醇二甲基丙烯酸酯、乙二醇、水和葡萄糖氧化酶的冷冻溶液进行辐射诱导聚合而合成的。这些聚合物是水凝胶，根据pH值或葡萄糖浓度，其含水量在60%至90%的范围内。水凝胶的膨胀和渗透性变化是由接触葡萄糖溶液引起的。葡萄糖氧化酶催化膜中葡萄糖氧化形成的葡萄糖酸降低了系统的pH值，从而导致膜的变化。还报道了这些膜在体外和体内对酶活性的保留情况。用不同化学配方制成的膜之间在性能上的巨大差异表明，具有人工胰腺所需性能特征的葡萄糖敏感膜可能是一个可以实现的目标。
