School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China.
School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China.
Poult Sci. 2024 Oct;103(10):104050. doi: 10.1016/j.psj.2024.104050. Epub 2024 Jun 28.
Brevibacillus laterosporus S62-9 has been shown to improve broiler growth performance and immunity. In the present study, we aimed to evaluate the effects of B. laterosporus S62-9 on the immunity and lipid metabolism of broilers by means of transcriptomic analysis. A total of 160 1-day-old broilers were randomly allocated to a S62-9 group, the diet of which was supplemented with 10 CFU/g B. laterosporus S62-9 daily, and a control group, which was not. After 42 d of feeding, the broilers in the S62-9 group had higher body mass (7.2%) and feed conversion ratio (5.19%) than the control group. Supplementation with B. laterosporus S62-9 resulted in lower serum total cholesterol and low-density lipoprotein-cholesterol concentrations and higher high-density lipoprotein-cholesterol concentrations. An analysis of the fatty acid composition of the broiler's thigh muscles revealed that the proportions of the unsaturated fatty acids myristoleic acid (C14:1) and arachidonic acid (C20:1) were higher for birds in the S62-9 group. Transcriptomic analysis also showed an upregulation of immunity-related genes in the S62-9 group. Gene Ontology functional enrichment analysis showed that the mitogen-activated protein kinase pathway was enriched in the liver, the defense response was enriched in the duodenum, and immunoglobulin-related entries were enriched in the jejunum of the S62-9 group. Furthermore, the expression of key genes involved in unsaturated fatty acid synthesis (SCD, encoding stearoyl-CoA desaturase) and fatty acid metabolism (HACD2, encoding 3-hydroxyacyl-CoA dehydratase 2) was upregulated in the liver, and the expression of genes associated with fat biosynthesis and accumulation, such as PLIN1, encoding perilipin 1, and FABP4, encoding fatty acid binding protein 4, was upregulated in the ileum of the birds in the S62-9 group. In summary, supplementation with B. laterosporus S62-9 could improve immune defense and the fatty acid metabolism of broiler chickens, thereby enhancing their disease resistance and promoting growth and development.
短芽孢杆菌 S62-9 已被证明能改善肉鸡的生长性能和免疫力。在本研究中，我们旨在通过转录组分析评估短芽孢杆菌 S62-9 对肉鸡免疫和脂质代谢的影响。将 160 只 1 日龄的肉鸡随机分为 S62-9 组和对照组，S62-9 组日粮中添加 10 CFU/g 的短芽孢杆菌 S62-9，对照组不添加。饲养 42d 后，S62-9 组肉鸡的体重（7.2%）和饲料转化率（5.19%）均高于对照组。补充短芽孢杆菌 S62-9 可降低血清总胆固醇和低密度脂蛋白胆固醇浓度，提高高密度脂蛋白胆固醇浓度。对肉鸡大腿肌肉脂肪酸组成的分析表明，S62-9 组肉鸡腿肌中豆蔻油酸（C14:1）和花生四烯酸（C20:1）等不饱和脂肪酸的比例较高。转录组分析还显示 S62-9 组免疫相关基因表达上调。基因本体论功能富集分析表明，S62-9 组肝脏中丝裂原活化蛋白激酶途径富集，十二指肠中防御反应富集，空肠中免疫球蛋白相关条目富集。此外，肝脏中不饱和脂肪酸合成（编码硬脂酰辅酶 A 去饱和酶）和脂肪酸代谢（编码 3-羟基酰基辅酶 A 脱水酶 2）关键基因的表达上调，与脂肪合成和积累相关的基因，如 perilipin 1 编码基因 PLIN1 和脂肪酸结合蛋白 4 编码基因 FABP4，在 S62-9 组肉鸡回肠中的表达上调。综上所述，补充短芽孢杆菌 S62-9 可以改善肉鸡的免疫防御和脂肪酸代谢，从而提高其抗病能力，促进生长发育。
