Xu Yanxue, Gao Xilan, Tao Tao, Ji Lili, Liu Maoping, Zhang Xicui, Xiao Dan
Institute of Advanced Study, Chengdu University, No. 2025, Chengluo Avenue, Chengdu, People's Republic of China.
College of Food and Bioengineering, Chengdu University, No. 2025, Chengluo Avenue, Chengdu, People's Republic of China.
Mikrochim Acta. 2024 Aug 6;191(9):518. doi: 10.1007/s00604-024-06574-z.
A nanocomposite of cobalt nanoparticle (CoNP) functionalized carbon nanotube (Co@CNT) was prepared and used to modify a glassy carbon electrode (Co@CNT/GCE). Characterization indicates the morphology of Co@CNT is CoNPs adhering on CNTs. With the nano-interface, Co@CNT provides large surface area, high catalytic activity, and efficient electron transfer, which makes Co@CNT/GCE exhibiting satisfactory electrochemical response toward quercetin (QC) and folic acid (FA). The optimum pH values for the detection of FA and QC are 7.0 and 3.0, respectively. The saturated absorption capacity (Γ*) and catalytic rate constant (k) of Co@CNT/GCE for QC and FA are calculated as 1.76 × 10, 3.94 × 10 mol∙cm and 3.04 × 10, 0.569 × 10 M∙s. The linear range for both FA and QC is estimated to be 5.0 nM-10 μM, and the LODs (3σ/s) were 2.30 nM and 2.50 nM, respectively. The contents of FA and QC in real samples determined by Co@CNT/GCE are comparable with the results determined by HPLC. The recoveries were in the range 90.5 ~ 114% and the total RSD was lower than 8.67%, which further confirms the reliability of the proposed electrode for practical use.
制备了一种钴纳米颗粒（CoNP）功能化碳纳米管的纳米复合材料（Co@CNT），并用于修饰玻碳电极（Co@CNT/GCE）。表征表明Co@CNT的形态是CoNP附着在CNT上。借助该纳米界面，Co@CNT提供了大表面积、高催化活性和高效的电子转移，这使得Co@CNT/GCE对槲皮素（QC）和叶酸（FA）表现出令人满意的电化学响应。检测FA和QC的最佳pH值分别为7.0和3.0。计算出Co@CNT/GCE对QC和FA的饱和吸附容量（Γ*）和催化速率常数（k）分别为1.76×10、3.94×10 mol∙cm和3.04×10、0.569×10 M∙s。FA和QC的线性范围估计均为5.0 nM - 10 μM，检测限（3σ/s）分别为2.30 nM和2.50 nM。用Co@CNT/GCE测定实际样品中FA和QC的含量与HPLC测定结果相当。回收率在90.5%至114%范围内，总相对标准偏差低于8.67%，这进一步证实了所提出电极实际应用的可靠性。
