HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, China.
Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou, China.
Immunogenetics. 2024 Dec;76(5-6):305-314. doi: 10.1007/s00251-024-01349-5. Epub 2024 Aug 6.
The Zhejiang Han population, a subgroup of the Southern Han ethnic group, resides in Zhejiang Province, situated on the southeast coast of China. In this study, we conducted HLA genotyping for 813 voluntary umbilical cord blood donors from the Zhejiang Han population, targeting 11 HLA loci, namely HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1, using the next-generation sequencing method. Our analysis of the alleles and haplotypes revealed a high degree of polymorphism within these loci. A total of 289 unique HLA alleles were identified, with the HLA-B locus exhibiting the most significant diversity, while HLA-DRB4 displayed the lowest variation. Due to the inherent limitations of the sequencing method, some unresolvable alleles in the specific loci, such as HLA-DRB1, HLA-DPA1, and HLA-DPB1, were assigned as G group designation. In our comprehensive analysis across all 11 HLA loci, a total of 1204 haplotypes were estimated. The distribution of these alleles was similar to those of the Chinese Southern Han population while highly different from the Caucasian population. These findings contribute to a deeper understanding of the genetic characteristics of HLA loci within the Chinese Southern Han population.
浙江汉族人群是南方汉族群体的一个分支，居住在中国东南沿海的浙江省。本研究采用高通量测序技术，对 813 名浙江汉族自愿捐献的脐带血样本进行了 11 个 HLA 基因座（HLA-A、B、C、DRB1、DRB3/4/5、DQA1、DQB1、DPA1 和 DPB1）的 HLA 基因分型。我们对这些等位基因和单倍型进行了分析，发现这些基因座具有高度多态性。共鉴定出 289 个独特的 HLA 等位基因，其中 HLA-B 基因座的多样性最为显著，而 HLA-DRB4 的变异最小。由于测序方法的固有局限性，某些特定基因座（如 HLA-DRB1、DPA1 和 DPB1）中的一些无法解决的等位基因被指定为 G 组。在我们对所有 11 个 HLA 基因座的综合分析中，共估计了 1204 个单倍型。这些等位基因的分布与中国南方汉族人群相似，但与高加索人群有很大的不同。这些发现有助于深入了解中国南方汉族人群中 HLA 基因座的遗传特征。
