Gil-González Aina Belén, Sjögren Lars L E, Bernfur Katja, Olsson Olof, Zambrano Jose Alfredo
Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Lund, Sweden.
Plant Breeding, Lantmännen, Svalöv, Sweden.
Front Plant Sci. 2024 Jul 23;15:1418658. doi: 10.3389/fpls.2024.1418658. eCollection 2024.
Oats, a highly nutritious cereal known for their health benefits, contain various macromolecules of significant biological value, including abundant and highly digestible proteins. Despite their importance, oat proteins have not been extensively studied. Here, we present a complete set of the expressed globulins genes, which code for the main storage protein in oats as well as their chromosomal positions.
Published expressed sequence tags for globulins were used as queries in the Sang oat genome. In addition, globulin proteins were fractionated from oat flour by solvent extraction based on differential solubility with other classes of cereal proteins. The protein fractions were separated by gel electrophoresis and analyzed by tandem mass spectrometry to confirm their identity and expression in seed.
In total 32 globulin gene sequences were identified on the oat genome. Out of these, the expression on RNA level could be confirmed and 27 were also detected as expressed proteins by MS. Our results provide the most extensive set of salt-soluble oat globulin sequences to date, paving the way for further understanding their implications for human nutrition. In addition, a simple methodology to fractionate oat proteins is presented.
燕麦是一种营养丰富的谷物，因其对健康有益而闻名，含有多种具有重要生物学价值的大分子物质，包括丰富且易于消化的蛋白质。尽管燕麦蛋白很重要，但尚未得到广泛研究。在此，我们展示了一组完整的表达球蛋白基因，这些基因编码燕麦中的主要贮藏蛋白及其染色体位置。
已发表的球蛋白表达序列标签用作查询桑燕麦基因组的检索词。此外，基于与其他类谷物蛋白的溶解性差异，通过溶剂萃取从燕麦粉中分离球蛋白。通过凝胶电泳分离蛋白质组分，并通过串联质谱分析以确认其在种子中的身份和表达。
在燕麦基因组上总共鉴定出32个球蛋白基因序列。其中，RNA水平的表达可以得到确认，并且通过质谱也检测到27个为表达蛋白。我们的结果提供了迄今为止最广泛的一组盐溶性燕麦球蛋白序列，为进一步了解它们对人类营养的影响铺平了道路。此外，还介绍了一种简单的燕麦蛋白分离方法。
