Complete chloroplast genomes of two species and the phylogenetic analysis in the tribe Pertyeae.

The genus DC. is one of the major groups within the tribe Pertyeae (Asteraceae). It comprises several important Chinese medicinal species. However, the phylogenetic position has undergone a long process of exploration. The complete chloroplast (cp) genome sequences data has not been employed in species identification and phylogeny of . In this study, the complete cp genomes of two species ( and ) were reported, followed by structural, comparative, and phylogenetic analyses within the tribe Peryteae. Both cp genomes displayed a typical quadripartite circular structure, with the LSC and SSC regions separated by the IR regions. The genomes were 152,959 () and 152,805 () base pairs (bp) long, with a GC content of 37.6%. They were highly conserved, containing 134 genes, including 87 protein-coding genes, 37 tRNA genes, 8 rRNA genes, and 2 pseudogenes ( and ). Moreover, thirteen highly polymorphic regions (e.g., , , , , and ) were identified, indicating their potential as DNA barcodes. The phylogenetic analysis confirmed the placement of in the tribe Pertyeae, revealing close relationships with the genera and . In comparison with , was more closely related to . This study lays a theoretical foundation for future research on species identification, population genetics, resource conservation, and sustainable utilization within and Pertyeae.