Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China.
J Agric Food Chem. 2024 Aug 21;72(33):18455-18464. doi: 10.1021/acs.jafc.4c02957. Epub 2024 Aug 7.
Siderophores are small molecule iron chelators. The entomopathogenic fungus produces a plethora of siderophores under iron-limiting conditions. In this study, a siderophore biosynthesis pathway, akin to the general pathway observed in filamentous fungi, was revealed in . Among the siderophore biosynthesis genes (SID), BbSidA was required for the production of most siderophores, and the SidC and SidD biosynthesis gene clusters were indispensable for the production of ferricrocin and fusarinine C, respectively. Biosynthesis genes play various roles in siderophore production, vegetative growth, stress resistance, development, and virulence, in which BbSidA plays the most important role. Accordingly, employs a cocktail of siderophores for iron metabolism, which is essential for fungal physiology and host interactions. This study provides the initial network for the genetic modification of siderophore biosynthesis, which not only aims to improve the efficacy of biocontrol agents but also ensures the efficient production of siderophores.
铁载体是小分子铁螯合剂。在缺铁条件下，昆虫病原真菌会产生大量铁载体。本研究在 中揭示了一条类似于丝状真菌中观察到的一般途径的铁载体生物合成途径。在铁载体生物合成基因（SID）中，BbSidA 是大多数铁载体产生所必需的，而 SidC 和 SidD 生物合成基因簇分别是产生铁血素和呋咱素 C 所必需的。生物合成基因在铁载体产生、营养生长、抗逆性、发育和毒力等方面发挥着不同的作用，其中 BbSidA 起着最重要的作用。因此， 利用铁载体鸡尾酒进行铁代谢，这对真菌生理学和宿主相互作用至关重要。本研究为铁载体生物合成的遗传修饰提供了初始网络，这不仅旨在提高生物防治剂的功效，而且还确保了铁载体的高效生产。
