Kopka Bartosz, Kost Bartłomiej, Pawlak Andrzej, Bąk-Sypień Irena, Brzeziński Marek, Tomaszewska Agata, Krupa Agnieszka, Jóźwiak Piotr, Basko Malgorzata
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.
BioMedChem Doctoral School of the University of Lodz and Lodz Institutes of the Polish Academy of Sciences, Matejki 21/23, 90-237 Lodz, Poland.
Soft Matter. 2024 Aug 22;20(33):6655-6667. doi: 10.1039/d4sm00615a.
In this study, porous networks were efficiently prepared by crosslinking hydrophilic poly(2-isopropenyl-2-oxazoline) (PiPOx) with dicarboxylic polyesters (HOOC-PLA-COOH or HOOC-PCL-COOH) in the presence of sodium chloride as a water-soluble porogen. Importantly, by using a relatively simple synthetic protocol, the resulting spongy materials were freely formed to the desired size and shape while maintaining stable dimensions. According to the SEM data, the porous 3D structure can be altered by the pore dimensions, which are dependent on the porogen crystal size. After porosity characterization, the mechanical properties were also evaluated uniaxial compression and tensile tests. The porous networks formed hydrogels with a high water absorption capacity. Finally, after showing cytocompatibility by the MTT assay, we also demonstrated the applicability of the porous hydrogels as scaffolds for cell cultivation. The presented results suggest that this type of hydrogels is a promising material for tissue engineering.
在本研究中，通过在作为水溶性致孔剂的氯化钠存在下，使亲水性聚（2-异丙烯基-2-恶唑啉）（PiPOx）与二羧酸聚酯（HOOC-PLA-COOH或HOOC-PCL-COOH）交联，有效地制备了多孔网络。重要的是，通过使用相对简单的合成方案，所得海绵状材料可自由形成所需的尺寸和形状，同时保持稳定的尺寸。根据扫描电子显微镜（SEM）数据，多孔三维结构可通过取决于致孔剂晶体尺寸的孔尺寸来改变。在孔隙率表征之后，还通过单轴压缩和拉伸试验评估了力学性能。所形成的多孔网络形成了具有高吸水能力的水凝胶。最后，通过MTT试验显示细胞相容性后，我们还证明了多孔水凝胶作为细胞培养支架的适用性。所呈现的结果表明，这种类型的水凝胶是用于组织工程的有前途的材料。
