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濒危物种狐猴(Daubentonia madagascariensis)的混合基因组组装。

A hybrid genome assembly of the endangered aye-aye (Daubentonia madagascariensis).

机构信息

Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85281, USA.

出版信息

G3 (Bethesda). 2024 Oct 7;14(10). doi: 10.1093/g3journal/jkae185.

Abstract

The aye-aye (Daubentonia madagascariensis) is the only extant member of the Daubentoniidae primate family. Although several reference genomes exist for this endangered strepsirrhine primate, the predominant usage of short-read sequencing has resulted in limited assembly contiguity and completeness, and no protein-coding gene annotations have yet been released. Here, we present a novel, fully annotated, chromosome-level hybrid de novo assembly for the species based on a combination of Oxford Nanopore Technologies long reads and Illumina short reads and scaffolded using genome-wide chromatin interaction data-a community resource that will improve future conservation efforts as well as primate comparative analyses.

摘要

狐猴(Daubentonia madagascariensis)是唯一现存的 Daubentoniidae 灵长类动物家族成员。尽管这种濒危的食虫目灵长类动物有几个参考基因组,但主要使用短读测序导致了有限的组装连续性和完整性,并且尚未发布任何蛋白质编码基因注释。在这里,我们基于 Oxford Nanopore Technologies 长读和 Illumina 短读的组合,以及使用全基因组染色质相互作用数据进行支架构建,提出了一个新的、完全注释的、染色体水平的狐猴从头杂交组装。这是一个社区资源,将改善未来的保护工作以及灵长类动物比较分析。

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