Department of Chemical and Biological Engineering, Monash University, Clayton, Melbourne, Victoria 3800, Australia.
School of Health and Biomedical Sciences, RMIT University, Bundoora, Melbourne, Victoria 3083, Australia.
ACS Appl Mater Interfaces. 2024 Aug 21;16(33):43329-43340. doi: 10.1021/acsami.4c08415. Epub 2024 Aug 7.
Investigating the interactions between nanomaterials and the cells they are likely to encounter in vivo is a critical aspect of designing nanomedicines for imaging and therapeutic applications. Immune cells such as dendritic cells, macrophages, and myeloid derived suppressor cells have a frontline role in the identification and removal of foreign materials from the body, with interactions shown to be heavily dependent on variables such as nanoparticle size, charge, and surface chemistry. Interactions such as cellular association or uptake of nanoparticles can lead to diminished functionality or rapid clearance from the body, making it critical to consider these interactions when designing and synthesizing nanomaterials for biomedical applications ranging from drug delivery to imaging and biosensing. We investigated the interactions between PEGylated organosilica nanoparticles and naturally endocytic immune cells grown from stem cells in murine bone marrow. Specifically, we varied the particle size from 60 nm up to 1000 nm and investigated the effects of size on immune cell association, activation, and maturation with these critical gatekeeper cells. These results will help inform future design parameters for in vitro and in vivo biomedical applications utilizing organosilica nanoparticles.
研究纳米材料与体内可能遇到的细胞之间的相互作用，是设计用于成像和治疗应用的纳米药物的关键方面。树突状细胞、巨噬细胞和髓系来源的抑制细胞等免疫细胞在识别和清除体内异物方面发挥着前沿作用，研究表明，这种相互作用严重依赖于纳米颗粒的大小、电荷和表面化学等变量。纳米颗粒与细胞的相互作用，如细胞的关联或摄取，可能导致其功能降低或从体内迅速清除，因此，在设计和合成用于从药物输送到成像和生物传感等生物医学应用的纳米材料时，必须考虑这些相互作用。我们研究了 PEG 化有机硅纳米颗粒与源自鼠骨髓干细胞的天然内吞免疫细胞之间的相互作用。具体来说，我们改变了粒径从 60nm 到 1000nm，并研究了粒径对免疫细胞关联、激活和成熟的影响，这些是关键的守门细胞。这些结果将有助于为利用有机硅纳米颗粒的体外和体内生物医学应用提供未来的设计参数。
