探讨麦卢卡蜂蜜对导致巴基斯坦败血症的多重耐药和广泛耐药伤寒沙门氏菌的抗菌疗效。
Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Typhi causing septicemia in Pakistan.
机构信息
Institute of Microbiology, Faculty of Life Sciences, Government College University Faisalabad, Faisalabad, 38000, Pakistan.
Department of Pathology, Ameer ud Din Medical College/Lahore General Hospital, Lahore, Pakistan.
出版信息
Future Microbiol. 2024;19(16):1377-1387. doi: 10.1080/17460913.2024.2384260. Epub 2024 Aug 7.
To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of Typhi. Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. MDR and XDR- Typhi was susceptible to azithromycin. These strains carried the H58, , , , and genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. Manuka honey could be an alternative option for treating Typhi infections.
目的
评估麦卢卡蜂蜜对多重耐药（MDR）和广泛耐药（XDR）伤寒临床分离株的疗效。
方法
使用 Bactec 血培养系统对临床分离株进行处理，通过 Vitek 2 进行鉴定和药敏试验，通过聚合酶链反应（PCR）检测抗生素耐药基因。微量肉汤稀释法评估麦卢卡蜂蜜的抗菌活性。MDR 和 XDR-伤寒对阿奇霉素敏感。这些菌株携带 H58、、、和基因。在 100%浓度的蜂蜜中，MDR（15-23mm）和 XDR（15-24mm）菌株的抑菌环直径分别为 15-23mm 和 15-24mm。18/50 株 MDR 和 14/50 株 XDR 分别在 3.125v/v%和 6.25v/v%的浓度下被抑制，在 6.25v/v%的浓度下被杀死。麦卢卡蜂蜜可能是治疗伤寒感染的一种替代选择。
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