Chaudhury A M, Smith G R
Mol Gen Genet. 1985;201(3):525-8. doi: 10.1007/BF00331350.
Induction of the SOS genes is required for efficient repair of damaged DNA in Escherichia coli. SOS induction by nalidixic acid or oxolinic acid, two inhibitors of DNA gyrase, requires the RecBC enzyme of E. coli. We report here that the nuclease activity of RecBC enzyme is not needed for SOS induction by these agents. We suggest that the unwinding activity of RecBC enzyme produces single-stranded DNA which activates the RecA protein to stimulate LexA repressor cleavage and SOS induction.
在大肠杆菌中，诱导SOS基因对于有效修复受损DNA是必需的。萘啶酸或恶喹酸这两种DNA促旋酶抑制剂诱导SOS反应需要大肠杆菌的RecBC酶。我们在此报告，这些试剂诱导SOS反应不需要RecBC酶的核酸酶活性。我们认为，RecBC酶的解旋活性产生单链DNA，激活RecA蛋白以刺激LexA阻遏物的切割和SOS反应的诱导。
