REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal; IFIMUP, Instituto de Física de Materiais Avançados, Nanotecnologia e Fotónica, Departamento de Física e Astronomia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4249-015 Porto, Portugal.
Food Chem. 2024 Dec 1;460(Pt 2):140733. doi: 10.1016/j.foodchem.2024.140733. Epub 2024 Aug 2.
Celery is a food allergen that must be included in the ingredient list of commercial food products in the European Union. This is a challenge for the food industry because of potential cross-contamination and undeclared ingredients because of their low concentrations. So, the food industry requires expedited high-performance analytical methods. The development, validation and application of a magnetic nanomaterial-based voltammetric immunosensor is reported to quantify a major celery allergen (Api g 1), achieving a low limit of detection (32 pg·mL, in a 40-μL sample). The applicability of the biosensor was evaluated by analysing twenty food products and the lowest Api g 1 content (1.1 ± 0.9 mg·kg) was quantified in a cooked sample. The selectivity of the method and the interference of similar fresh products (e.g., parsley, basil) were evaluated. This portable and easy-to-use biosensor can be a fit-for-purpose solution to tackle a major problem for the food industry.
芹菜是一种食物过敏原，必须包含在欧盟商业食品产品的成分清单中。由于潜在的交叉污染和未申报的成分（由于其浓度低），这对食品行业来说是一个挑战。因此，食品行业需要加速开发高性能分析方法。本文报道了一种基于磁性纳米材料的伏安免疫传感器的开发、验证和应用，用于定量测定一种主要的芹菜过敏原（Api g 1），检测限低至 32 pg·mL（40 μL 样品）。通过分析 20 种食品产品来评估生物传感器的适用性，并在一份煮熟的样品中定量检测到最低的 Api g 1 含量（1.1±0.9 mg·kg）。评估了该方法的选择性和类似新鲜产品（如欧芹、罗勒）的干扰。这种便携式、易于使用的生物传感器可以成为解决食品行业主要问题的合适解决方案。
