Zhan Yuejuan, Zhang Qiran, Wang Wenyang, Liang Wenyi, Wang Chengdi
Department of Pulmonary and Critical Care Medicine, Targeted Tracer Research and Development Laboratory, State Key Laboratory of Respiratory Health and Multimorbidity, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
West China School of Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
Chin Med J (Engl). 2024 Aug 6. doi: 10.1097/CM9.0000000000003095.
Tuberculosis (TB) has one of the highest mortality rates among infectious diseases worldwide. The immune response in the host after infection is proposed to contribute significantly to the progression of TB, but the specific mechanisms involved remain to be elucidated. Single-cell RNA sequencing (scRNA-seq) provides unbiased transcriptome sequencing of large quantities of individual cells, thereby defining biological comprehension of cellular heterogeneity and dynamic transcriptome state of cell populations in the field of immunology and is therefore increasingly applied to lung disease research. Here, we first briefly introduce the concept of scRNA-seq, followed by a summarization on the application of scRNA-seq to TB. Furthermore, we underscore the potential of scRNA-seq for clinical biomarker exploration, host-directed therapy, and precision therapy research in TB and discuss the bottlenecks that need to be overcome for the broad application of scRNA-seq to TB-related research.
结核病（TB）在全球传染病中死亡率位居前列。宿主感染后的免疫反应被认为对结核病的进展有显著影响，但其具体机制仍有待阐明。单细胞RNA测序（scRNA-seq）能对大量单个细胞进行无偏转录组测序，从而在免疫学领域定义细胞异质性和细胞群体动态转录组状态的生物学理解，因此越来越多地应用于肺部疾病研究。在此，我们首先简要介绍scRNA-seq的概念，接着总结scRNA-seq在结核病中的应用。此外，我们强调scRNA-seq在结核病临床生物标志物探索、宿主导向治疗和精准治疗研究方面的潜力，并讨论scRNA-seq广泛应用于结核病相关研究需要克服的瓶颈。
