布基纳法索瓦加杜古患者中登革热病毒3型占主导地位。

Predominance of DENV-3 among patients in Ouagadougou, Burkina Faso.

作者信息

Ouattara Abdoul Karim, Toyin Bello Shoukrat Ohuwa, Ouédraogo Alphonsine, Traoré Lassina, Djigma Florencia Wendkuuni, Simporé Jacques

机构信息

Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), BP 364 Ouagadougou 01, Burkina Faso.

Université Norbert Zongo/ Centre Universitaire de Manga, BP 376 Koudougou, Burkina Faso.

出版信息

J Vector Borne Dis. 2024 Jul 7. doi: 10.4103/JVBD.JVBD_68_24.

DOI:10.4103/JVBD.JVBD_68_24

PMID:39113365

Abstract

BACKGROUND OBJECTIVES

Dengue is an emerging vector-borne viral disease in tropical and subtropical areas such as Burkina Faso that experienced dengue outbreak in, 2013, 2016, 2017 and more recently in 2023. This study aimed to determine the seroprevalence and dengue serotype in suspected patients in Ouagadougou, Burkina Faso.

METHODS

The study was conducted during October and November 2023 and included suspected febrile patients seen at HOSCO and CERBA. Plasma or serum samples were used for the detection of non-structural proteins (NS1) and IgM and IgG antibodies against the dengue virus using SD Bioline Dengue Duo rapid detection kit. Viral RNA was extracted using the QIAamp Viral RNA Mini Kit and dengue serotypes were determined by real-time RT-PCR using the Dengue Real-TM Genotype kit.

RESULTS

The study population consisted of 896 patients, including 397 (44.3%) men and 499 (55.7%) women. Dengue seroprevalence was 16.5% (148/896) with 14.1% (126/896) of patients positive for the NS1 antigen, 1.3% (12/896) positive for IgM, and 2.7% (24/896) positive for IgG. Serotyping among 40 out of 45 positive patients revealed 77.5% (31/40) DENV-3, 17.5% (7/40) DENV-1, and 5.0% (2/40) DENV-2.

INTERPRETATION CONCLUSION

The present study report a high seroprevalence of dengue virus infection among patients during the months considered as the peak of infection in Burkina Faso. The results revealed a predominance of DENV-3. Continuous surveillance of dengue virus serotypes circulating in Burkina Faso is crucial.

摘要

背景目的

登革热是一种在热带和亚热带地区出现的媒介传播病毒性疾病，如布基纳法索在2013年、2016年、2017年以及最近在2023年都经历了登革热疫情。本研究旨在确定布基纳法索瓦加杜古疑似患者中的血清阳性率和登革热血清型。

方法

该研究于2023年10月和11月进行，纳入了在HOSCO和CERBA就诊的疑似发热患者。使用SD Bioline登革热双联快速检测试剂盒，将血浆或血清样本用于检测非结构蛋白（NS1）以及抗登革热病毒的IgM和IgG抗体。使用QIAamp病毒RNA迷你试剂盒提取病毒RNA，并使用登革热Real-TM基因型试剂盒通过实时RT-PCR确定登革热血清型。

结果

研究人群包括896名患者，其中男性397名（44.3%），女性499名（55.7%）。登革热血清阳性率为16.5%（148/896），NS1抗原阳性患者为14.1%（126/896），IgM阳性为1.3%（12/896），IgG阳性为2.7%（24/896）。45名阳性患者中的40名进行血清分型显示，77.5%（31/40）为DENV-3，17.5%（7/40）为DENV-1，5.0%（2/40）为DENV-2。