Klinder A, Möws F, Ziebart J, Su Y, Gabler C, Jonitz-Heincke A, van Rienen U, Ellenrieder M, Bader R
Research Laboratory for Biomechanics and Implant Technology, Department of Orthopedics, Rostock University Medical Center, Rostock, Germany.
Institute of General Electrical Engineering, University of Rostock, Rostock, Germany.
Front Bioeng Biotechnol. 2024 Jul 24;12:1395715. doi: 10.3389/fbioe.2024.1395715. eCollection 2024.
Electrical stimulation has been used as a promising approach in bone repair for several decades. However, the therapeutic use is hampered by inconsistent results due to a lack of standardized application protocols. Recently, electrical stimulation has been considered for the improvement of the osseointegration of dental and endoprosthetic implants. In a pilot study, the suitability of a specifically developed device for electrical stimulation was assessed. Here, the impact of alternating electric fields on implant osseointegration was tested in a gap model using New Zealand White Rabbits. Stimulation parameters were transmitted to the device via a radio transceiver, thus allowing for real-time monitoring and, if required, variations of stimulation parameters. The effect of electrical stimulation on implant osseointegration was quantified by the bone-implant contact (BIC) assessed by histomorphometric (2D) and µCT (3D) analysis. Direct stimulation with an alternating electric potential of 150 mV and 20 Hz for three times a day (45 min per unit) resulted in improved osseointegration of the triangular titanium implants in the tibiae of the rabbits. The ratio of bone area in histomorphometry (2D analysis) and bone volume (3D analysis) around the implant were significantly increased after stimulation compared to the untreated controls at sacrifice 84 days after implantation. The developed experimental design of an electrical stimulation system, which was directly located in the defect zone of rabbit tibiae, provided feedback regarding the integrity of the stimulation device throughout an experiment and would allow variations in the stimulation parameters in future studies. Within this study, electrical stimulation resulted in enhanced implant osseointegration. However, direct electrical stimulation of bone tissue requires the definition of dose-response curves and optimal duration of treatment, which should be the subject of subsequent studies.
几十年来，电刺激一直被用作骨修复中一种很有前景的方法。然而，由于缺乏标准化的应用方案，其治疗效果并不一致，这阻碍了其临床应用。近年来，电刺激被认为可用于改善牙种植体和内置假体的骨整合。在一项初步研究中，评估了一种专门开发的电刺激装置的适用性。在此，利用新西兰白兔，在间隙模型中测试了交变电场对种植体骨整合的影响。刺激参数通过无线电收发器传输到该装置，从而实现实时监测，并在需要时改变刺激参数。通过组织形态计量学（二维）和显微计算机断层扫描（三维）分析评估骨-种植体接触（BIC），以量化电刺激对种植体骨整合的影响。每天三次（每次45分钟）施加150mV和20Hz的交变电势直接刺激，可改善兔胫骨中三角形钛种植体的骨整合。与植入后84天处死时的未治疗对照组相比，刺激后种植体周围组织形态计量学（二维分析）中的骨面积和骨体积（三维分析）比例显著增加。所开发的电刺激系统实验设计直接位于兔胫骨的缺损区，在整个实验过程中提供了有关刺激装置完整性的反馈，并将允许在未来的研究中改变刺激参数。在本研究中，电刺激增强了种植体的骨整合。然而，对骨组织的直接电刺激需要确定剂量反应曲线和最佳治疗持续时间，这应是后续研究的主题。
