Tabassum Fatema, Islam Sheikh Nazrul, Tuz-Zohora Fatema, Hasan Choudhury Mahmood, Rahman Khondaker Miraz, Ahsan Monira
Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka, 1000, Bangladesh.
Department of Pharmacy, Stamford University Bangladesh, 51 Siddheswari Rd, Dhaka, 1217, Bangladesh.
Heliyon. 2024 Jul 11;10(14):e34508. doi: 10.1016/j.heliyon.2024.e34508. eCollection 2024 Jul 30.
A new 2-quinolone alkaloid, iso-oligophyline (), and two very unusual C terpenoids, proposed names ravespanol () and ravespanone (), along with two known compounds, β-sitosterol (), and methyl linoleate (), were isolated from the leaf extract of . Methyl linoleate constitutes the first report of isolation from this species. We have already reported the isolation of atanine (), oligophyline (), ravenoline (8), and arborinine ) from the plant. Based on nuclear magnetic resonance (NMR) spectroscopy and mass spectrometric analysis, the structure of the isolated chemicals was determined. The crude fractions and four compounds (,, and ) were evaluated for a cytotoxicity study on a panel of six human stomach cancer cell lines (SCL, SCL-6, SCL-37'6, SCL-9, K-3, N21) by MTT assay. Among the plant extracts and isolated compounds, petroleum ether fraction and compound exhibited the highest cytotoxic activity against SCL and SCL-6 cells, where the IC values were 17.9 and 16.56 μM, respectively.
从[植物名称]的叶提取物中分离出一种新的2-喹诺酮生物碱异寡叶碱（）、两种非常特殊的C类萜，暂定名为瑞夫斯帕醇（）和瑞夫斯帕酮（），以及两种已知化合物β-谷甾醇（）和亚油酸甲酯（）。亚油酸甲酯是首次从该物种中分离得到的报道。我们已经报道过从该植物中分离出阿塔宁（）、寡叶碱（）、鸦藤灵（8）和乔木宁（）。基于核磁共振（NMR）光谱和质谱分析确定了分离出的化学物质的结构。通过MTT法对粗提物和四种化合物（、、和）在六种人胃癌细胞系（SCL、SCL-6、SCL-37'6、SCL-9、K-3、N21）上进行了细胞毒性研究评估。在植物提取物和分离出的化合物中，石油醚提取物和化合物对SCL和SCL-6细胞表现出最高的细胞毒性活性，其IC值分别为17.9和16.56μM。
