使用荧光激活粒子分选法纯化相分离的 p62 体的方法。
Method to Purify Phase-Separated p62 Bodies Using Fluorescence-Activated Particle Sorting.
机构信息
Department of Physiology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan.
Department of Molecular Cell Biology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
出版信息
Methods Mol Biol. 2024;2845:191-196. doi: 10.1007/978-1-0716-4067-8_15.
p62 bodies are ubiquitin-positive cytoplasmic condensates formed by liquid-liquid phase separation. They are targeted by selective autophagy and play important roles in intracellular quality control and stress responses. However, little is known about their constituents. In this chapter, we describe a method for purifying p62 bodies using fluorescence-activated particle sorting. This method contributes to the identification of novel components of p62 bodies under various physiological and stress conditions.
p62 体是由液-液相分离形成的泛素阳性细胞质凝聚物。它们被选择性自噬靶向，并在细胞内质量控制和应激反应中发挥重要作用。然而，它们的组成成分知之甚少。在本章中，我们描述了一种使用荧光激活粒子分选纯化 p62 体的方法。该方法有助于在各种生理和应激条件下鉴定 p62 体的新成分。
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