Department of Mathematics & Natural Sciences, School of Data and Sciences, BRAC University, Dhaka, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
Mol Biol Rep. 2024 Aug 8;51(1):893. doi: 10.1007/s11033-024-09707-y.
In Bangladesh, only a fraction of prostate cancer patients are diagnosed annually due to lack of symptom awareness and screening challenges, resulting in high mortality. Aiming to improve screening methods, we evaluated X-ray cross-complementing gene 1 (XRCC1) Arg194Gln and Xeroderma pigmentosum group D (XPD) Lys751Gln polymorphisms to determine their relevance as potential markers for predicting prostate cancer risk, severity and clinical parameters in Bangladeshi population.
This study included 132 prostate cancer patients and 135 healthy controls. Genotype analysis was done from blood samples by the PCR-RFLP method. The XRCC1 Trp/Trp genotype was associated with prostate cancer (OR = 5.51; 95% CI = 1.13-26.78; p-value = 0.03) compared to Arg/Arg genotype. No significant association was found between the XPD variants and prostate cancer risk. The XRCC1 Trp/Trp genotype increased prostate cancer risk in smokers and non-smokers but was statistically non-significant. In individuals without a family history of cancer, the XRCC1 Trp/Trp genotype had a non-significant 4.64-fold higher risk (OR=4.64; 95% CI = 0.88-24.36; p-value = 0.07), while the XPD Gln/Gln had a 2.66-fold non-significant higher risk (OR=2.66; 95% CI = 0.88-8.10; p-value = 0.09). The XRCC1 Trp/Trp variant was associated with hematuria risk, higher mean serum creatinine, and mean prostate-specific antigen (PSA) levels in prostate cancer patients. The XPD Gln/Gln variant was only associated with higher mean serum creatinine levels.
Our findings suggest that XRCC1 screening may be used as a biomarker for prostate cancer to improve early diagnosis in Bangladesh.
在孟加拉国，由于缺乏症状意识和筛查挑战，每年只有一小部分前列腺癌患者被诊断出来，导致死亡率很高。为了改善筛查方法，我们评估了 X 射线修复交叉互补基因 1（XRCC1）Arg194Gln 和着色性干皮病组 D（XPD）Lys751Gln 多态性，以确定它们作为预测孟加拉国人群前列腺癌风险、严重程度和临床参数的潜在标志物的相关性。
本研究包括 132 例前列腺癌患者和 135 例健康对照者。通过 PCR-RFLP 法从血液样本中进行基因型分析。与 Arg/Arg 基因型相比，XRCC1 Trp/Trp 基因型与前列腺癌相关（OR=5.51；95%CI=1.13-26.78；p 值=0.03）。XPD 变体与前列腺癌风险之间没有显著关联。XRCC1 Trp/Trp 基因型增加了吸烟者和不吸烟者的前列腺癌风险，但无统计学意义。在没有癌症家族史的个体中，XRCC1 Trp/Trp 基因型的风险增加了 4.64 倍，但无统计学意义（OR=4.64；95%CI=0.88-24.36；p 值=0.07），而 XPD Gln/Gln 则增加了 2.66 倍（OR=2.66；95%CI=0.88-8.10；p 值=0.09）。XRCC1 Trp/Trp 变体与血尿风险、前列腺癌患者更高的平均血清肌酐和前列腺特异性抗原（PSA）水平相关。XPD Gln/Gln 变体仅与更高的平均血清肌酐水平相关。
我们的研究结果表明，XRCC1 筛查可作为前列腺癌的生物标志物，以提高孟加拉国的早期诊断。
