Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
Methods Mol Biol. 2024;2841:199-205. doi: 10.1007/978-1-0716-4059-3_19.
Biomolecular condensates are triggered by multivalent interactions conferred by the intrinsically disordered regions and/or interacting domains within the constituents. While light microscopy has provided powerful tools to study the dynamics of intracellular condensates, electron microscopy (EM) gives more detailed insights into their ultrastructure and spatial connectivity with membrane system. In this chapter, we describe the methods for detecting the membraneless condensates in plant cells by high-pressure freezing -based EM coupled with immuno-gold labeling and correlative light electron microscopy techniques, which may benefit researchers in future studies.
生物分子凝聚物是由组成成分中的无序区域和/或相互作用结构域赋予的多价相互作用引发的。虽然显微镜已为研究细胞内凝聚物的动力学提供了强大的工具，但电子显微镜 (EM) 则更深入地了解其超微结构和与膜系统的空间连接。在本章中，我们描述了通过高压冷冻-EM 结合免疫金标记和相关的光电子显微镜技术检测植物细胞中无膜凝聚物的方法，这可能有助于未来研究人员的研究。
