Engineering Research Centre of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, PR China.
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, the Republic of Korea.
Colloids Surf B Biointerfaces. 2024 Dec;244:114142. doi: 10.1016/j.colsurfb.2024.114142. Epub 2024 Aug 6.
Hyperglycemia provides a favorable breeding ground for bacteria, resulting in repeated and persistent inflammation of wounds and prolonged healing processes. In this study, platinum (Pt) nanoparticles (NPs) and glucose oxidase (GOx) were decorated on the surface of camelina lipid droplets (OB) linked with hFGF2, forming PGOB through in situ reduction of Pt ions and electrostatic adsorption, respectively. PGOB exhibits cascade enzyme catalytic activity, which can be activated by glucose in diabetic wound tissues. Specifically, GOx on PGOB catalyzes glucose into hydrogen peroxide, which can further decompose into hydroxyl radicals that have higher toxicity for bacterial inactivation. Additionally, glucose decomposition creates a low pH microenvironment, facilitating the cascade catalytic activity that ensures better bacterial suppression within the wound tissues. Furthermore, hFGF2 promotes the proliferation and migration of fibroblasts. Both in vitro and in vivo experiments confirm that PGOB effectively accelerates wound healing processes through bacteria inactivation and tissue regeneration. This study has developed an alternative strategy for glucose-triggered synergistic cascade therapy for diabetic wounds.
高血糖为细菌提供了有利的滋生环境，导致伤口反复且持续发炎，并延长愈合过程。在这项研究中，铂（Pt）纳米粒子（NPs）和葡萄糖氧化酶（GOx）被修饰在骆驼蓬脂质体（OB）的表面上，通过 Pt 离子的原位还原和静电吸附分别形成 PGOB。PGOB 表现出级联酶催化活性，可以被糖尿病伤口组织中的葡萄糖激活。具体来说，PGOB 上的 GOx 催化葡萄糖生成过氧化氢，过氧化氢可以进一步分解为羟基自由基，对细菌失活具有更高的毒性。此外，葡萄糖分解会产生低 pH 微环境，有利于级联催化活性，从而确保在伤口组织内更好地抑制细菌。此外，hFGF2 促进成纤维细胞的增殖和迁移。体外和体内实验均证实，PGOB 通过细菌失活和组织再生，有效地加速了伤口愈合过程。这项研究为糖尿病伤口的葡萄糖触发协同级联治疗开发了一种替代策略。
