State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun 130021, PR China.
J Colloid Interface Sci. 2023 Dec;651:319-333. doi: 10.1016/j.jcis.2023.07.139. Epub 2023 Jul 24.
Diabetes-related chronic wounds characterized by hyperglycemia and weak alkaline milieu provide numerous advantages for bacteria growth and biofilm formation, setting a myriad of stumbling blocks for wound healing. Therefore, reshaping the spatially and temporally pathological wound microenvironment against bacterial infection is critical to rescue stalled healing progress in diabetes-related chronic wounds. Herein, we demonstrate on the room-temperature construction of a glucose oxidase (GOx)-mimicking and peroxidase (POD)-mimicking dual-nanozymes catalytic cascade system upon the partial reduction of Fe to Fe and the deposition of Au nanoparticles, simultaneously. The as-prepared dual-nanozymes catalytic cascade system possesses the capabilities of reshaping the pathological microenvironments of diabetic wound via glucose consumption and acidification, leading to amplified catalytic cascade activities for sterilization. On the one hand, the GOx-mimicking enzymatic activity of the catalytic cascade system can not only deplete glucose and acidize wound milieu to inhibit bacteria growth, but also utilize the weak alkaline milieu of diabetic wound to provide sufficient HO and a favorable pH for subsequent OH generation. On the other hand, the POD-mimicking enzymatic activity of the catalytic cascade system can continuously produce OH for sterilization under the weak acidic milieu in the presence of abundant HO. Benefiting from the simply and mild preparation process and the excellent dual-nanozymes catalytic cascade activities under the deliberate evolved milieus of diabetes-related chronic wounds, our catalytic cascade system exhibits the promising healing effect and clinical translation potential against diabetic wound infection.
糖尿病相关的慢性创面的特征为高血糖和弱碱性环境，这为细菌生长和生物膜形成提供了诸多优势，给创面愈合造成了重重阻碍。因此，重塑针对细菌感染的时空病理性创面微环境对于挽救糖尿病相关慢性创面愈合停滞至关重要。在此，我们展示了在室温条件下，通过部分还原 Fe 为 Fe 和沉积 Au 纳米颗粒，构建了葡萄糖氧化酶（GOx）模拟和过氧化物酶（POD）模拟的双纳米酶级联催化系统。所制备的双纳米酶级联催化系统具有通过消耗葡萄糖和酸化来重塑糖尿病创面病理性微环境的能力，从而放大了杀菌的催化级联活性。一方面，催化级联系统的 GOx 模拟酶活性不仅可以消耗葡萄糖并酸化创面微环境以抑制细菌生长，还可以利用糖尿病创面的弱碱性环境为随后的 OH 生成提供充足的 HO 和有利的 pH。另一方面，在存在大量 HO 的弱酸性环境中，催化级联系统的 POD 模拟酶活性可以持续产生 OH 进行杀菌。受益于简单温和的制备工艺以及在糖尿病相关慢性创面刻意演变的微环境下卓越的双纳米酶级联催化活性，我们的催化级联系统在治疗糖尿病创面感染方面表现出了有前景的愈合效果和临床转化潜力。
