Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK.
Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy-en-Josas, France.
Bioresour Technol. 2024 Oct;410:131232. doi: 10.1016/j.biortech.2024.131232. Epub 2024 Aug 6.
Applying low-cost substrate is critical for sustainable bioproduction. Co-culture of phototrophic and heterotrophic microorganisms can be a promising solution as they can use CO and light as feedstock. This study aimed to create a light-driven consortium using a marine cyanobacterium Synechococcus sp. PCC 7002 and an industrial yeast Yarrowia lipolytica. First, the cyanobacterium was engineered to accumulate and secrete sucrose by regulating the expression of genes involved in sucrose biosynthesis and transport, resulting in 4.0 g/L of sucrose secretion. Then, Yarrowia lipolytica was engineered to efficiently use sucrose and produce β-caryophyllene that has various industrial applications. Then, co- and sequential-culture were optimized with different induction conditions and media compositions. A maximum β-caryophyllene yield of 14.1 mg/L was obtained from the co-culture. This study successfully established an artificial light-driven consortium based on a marine cyanobacterium and Y. lipolytica, and provides a foundation for sustainable bioproduction from CO and light through co-culture systems.
应用低成本基质对于可持续的生物生产至关重要。光合和异养微生物的共培养可以是一种很有前途的解决方案，因为它们可以利用 CO 和光作为原料。本研究旨在利用海洋蓝藻 Synechococcus sp. PCC 7002 和工业酵母 Yarrowia lipolytica 创建一个光驱动的联合体。首先，通过调节参与蔗糖生物合成和运输的基因的表达，工程化蓝藻积累和分泌蔗糖，导致 4.0 g/L 的蔗糖分泌。然后，工程化改造 Yarrowia lipolytica 以有效利用蔗糖并生产具有各种工业应用的 β-石竹烯。然后，优化了不同诱导条件和培养基组成的共培养和顺序培养。从共培养中获得了 14.1 mg/L 的最大 β-石竹烯产量。本研究成功地基于海洋蓝藻和 Y. lipolytica 建立了一个人工光驱动联合体，为通过共培养系统从 CO 和光进行可持续的生物生产提供了基础。
