Ornvold K, Nielsen M H, Clausen N
Acta Pathol Microbiol Immunol Scand A. 1985 Nov;93(6):311-6.
Fourteen cases of disseminated histiocytosis x (HX) from a 15 year period were studied clinicopathologically. Morbidity and mortality were comparable to that of previous reports on disseminated HX. S-100 protein, recently established as a HX marker, was demonstrated immunohistochemically in the cytoplasm and the nuclei of the HX cells of 12 examined cases. Neuron specific enolase (NSE) positive material was found in a minority of the cells of 2 cases. Cytoplasmic lysozyme was present in the mononuclear cells accompanying the HX cells in all examined cases. These results show that immunohistochemical demonstration of S-100 protein and lysozyme can be successfully applied to formalin-fixed, paraffin embedded tissue after storage at room temperature for as long as 15 years. The presence of cytoplasmic NSE positivity in the lesions from 2 patients was surprising and has not previously been observed in HX. This finding suggests an antigenic heterogenicity between cases with the disease of unknown prognostic significance. Nor did the presence of lysozyme in the lesions from patients with acute as well as chronic disease yield any prognostic information.
对15年间的14例播散性组织细胞增多症X（HX）病例进行了临床病理研究。发病率和死亡率与之前关于播散性HX的报告相当。S-100蛋白最近被确立为HX的标志物，在12例接受检查的病例中，通过免疫组织化学方法在HX细胞的细胞质和细胞核中均检测到该蛋白。在2例病例的少数细胞中发现神经元特异性烯醇化酶（NSE）阳性物质。在所有接受检查的病例中，伴随HX细胞的单核细胞中均存在细胞质溶菌酶。这些结果表明，S-100蛋白和溶菌酶的免疫组织化学检测可成功应用于在室温下保存长达15年的福尔马林固定、石蜡包埋组织。2例患者病变中细胞质NSE阳性的存在令人惊讶，此前在HX中未曾观察到。这一发现提示了该疾病病例之间存在抗原异质性，其预后意义不明。急性和慢性疾病患者病变中溶菌酶的存在也未提供任何预后信息。
