Ide F, Iwase T, Saito I, Umemura S, Nakajima T
Cancer. 1984 Feb 15;53(4):917-21. doi: 10.1002/1097-0142(19840215)53:4<917::aid-cncr2820530417>3.0.co;2-7.
The cellular nature of the proliferating histiocytes in 6 cases of histiocytosis X was studied immunohistochemically and ultrastructurally. Immunohistochemically, S-100 protein was detected both in the cytoplasm and the nuclei of histiocytosis X cells as well as Langerhans cells in normal oral epithelium. These cells were always negative for lysozyme, alpha 1-antitrypsin, alpha 1-antichymotrypsin and immunoglobulins. S-100 protein was not detected in lysozyme-positive histiocytes and multinucleated giant cells often showed the signs of phagocytosis. Thus, S-100 protein appears to be a useful immunohistochemical marker for histiocytosis X cells. Ultrastructurally, Birbeck granules noticed in histiocytosis X cells were never seen in the phagocytic histiocytes with numerous lysosomes and phagosomes. These results emphasized the heterogeneous nature of the proliferating histiocytes involved in the lesions. Since histiocytosis X cells share characteristics, not only ultrastructurally but also immunohistochemically, with Langerhans cells, the hypothesis that histiocytosis X may be fundamentally an abnormal proliferation of Langerhans cells has been further supported.
对6例组织细胞增多症X中增殖性组织细胞的细胞性质进行了免疫组织化学和超微结构研究。免疫组织化学显示，组织细胞增多症X细胞以及正常口腔上皮中的朗格汉斯细胞的细胞质和细胞核中均检测到S-100蛋白。这些细胞对溶菌酶、α1-抗胰蛋白酶、α1-抗糜蛋白酶和免疫球蛋白始终呈阴性。在溶菌酶阳性的组织细胞中未检测到S-100蛋白，多核巨细胞常显示吞噬迹象。因此，S-100蛋白似乎是组织细胞增多症X细胞的一种有用的免疫组织化学标志物。超微结构上，在组织细胞增多症X细胞中发现的伯贝克颗粒在具有大量溶酶体和吞噬体的吞噬性组织细胞中从未见过。这些结果强调了病变中增殖性组织细胞的异质性。由于组织细胞增多症X细胞不仅在超微结构上，而且在免疫组织化学上与朗格汉斯细胞具有共同特征，这进一步支持了组织细胞增多症X可能根本上是朗格汉斯细胞异常增殖的假说。
