Thomas D S, Rose A H
Arch Microbiol. 1979 Jul;122(1):49-55. doi: 10.1007/BF00408045.
Incorporation of ethanol (1.0 or 1.25 M) into exponential-phase cultures of Saccharomyces cerevisiae NCYC 366 growing anaerobically in a medium supplemented with ergosterol and an unsaturated fatty acid caused a retardation in growth rat, which was greater when the medium contained oleic rather than linoleic acid. Ethanol incorporation led to an immediate drop in growth rate, and ethanol-containing cultures grew at the slower rate for at least 10 h. Incorporation of ethanol (0.5 M) into buffered (pH 4.5) cell suspensions containing D-[6-3H] glucose, D-[1-14C] glucosamine, L-[U-14C] lysine or arginine, or KH232PO4 lowered the rate of solute accumulation by cells. Rates of accumulation of glucose, lysine and arginine were retarded to a greater extent when cells had been grown in the presence of oleic rather than linoleic acid. This difference was not observed with accumulation of phosphate. Ethanol was extracted from exponential-phase cells by four different methods. Cells grown in the presence of linoleic acid contained a slightly, but consistently, lower concentration of ethanol than cells grown in oleic acid-containing medium. The ethanol concentration in cells was 5-7 times greater than that in the cell-free medium.
将乙醇（1.0或1.25 M）加入到在补充了麦角固醇和一种不饱和脂肪酸的培养基中厌氧生长的酿酒酵母NCYC 366指数生长期培养物中，导致生长速率减慢，当培养基中含有油酸而非亚油酸时，这种减慢更为明显。加入乙醇导致生长速率立即下降，含乙醇的培养物以较慢的速率生长至少10小时。将乙醇（0.5 M）加入到含有D-[6-³H]葡萄糖、D-[1-¹⁴C]葡糖胺、L-[U-¹⁴C]赖氨酸或精氨酸或KH₂³²PO₄的缓冲（pH 4.5）细胞悬液中，降低了细胞溶质积累的速率。当细胞在油酸而非亚油酸存在下生长时，葡萄糖、赖氨酸和精氨酸的积累速率受到的抑制更大。对于磷酸盐的积累未观察到这种差异。通过四种不同方法从指数生长期细胞中提取乙醇。在亚油酸存在下生长的细胞中乙醇浓度略低于但始终低于在含油酸培养基中生长的细胞。细胞中的乙醇浓度比无细胞培养基中的乙醇浓度高5至7倍。
