State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
BMC Plant Biol. 2024 Aug 9;24(1):759. doi: 10.1186/s12870-024-05427-4.
Populus spp. is a crucial fast-growing and productive tree species extensively cultivated in the mid-latitude plains of the world. However, the impact of intensive cultivation management on gene expression in plantation remains largely unexplored.
Precision water and fertilizer-intensive management substantially increased key enzyme activities of nitrogen transport, assimilation, and photosynthesis (1.12-2.63 times than CK) in Populus × euramericana 'Neva' plantation. Meanwhile, this management approach had a significant regulatory effect on the gene expression of poplar plantations. 1554 differential expression genes (DEGs)were identified in drip irrigation (ND) compared with conventional irrigation. Relative to ND, 2761-4116 DEGs, predominantly up-regulated, were identified under three drip fertilization combinations, among which 202 DEGs were mainly regulated by fertilization. Moreover, drip irrigation reduced the expression of cell wall synthesis-related genes to reduce unnecessary water transport. Precision drip and fertilizer-intensive management promotes the synergistic regulation of carbon and nitrogen metabolism and up-regulates the expression of major genes in nitrogen transport and assimilation processes (5 DEGs), photosynthesis (15 DEGs), and plant hormone signal transduction (11 DEGs). The incorporation of trace elements further enhanced the up-regulation of secondary metabolic process genes. In addition, the co-expression network identified nine hub genes regulated by precision water and fertilizer-intensive management, suggesting a pivotal role in regulating the growth of poplar.
Precision water and fertilizer-intensive management demonstrated the ability to regulate the expression of key genes and transcription factor genes involved in carbon and nitrogen metabolism pathways, plant hormone signal transduction, and enhance the activity of key enzymes involved in related processes. This regulation facilitated nitrogen absorption and utilization, and photosynthetic abilities such as light capture, light transport, and electron transport, which faintly synergistically regulate the growth of poplar plantations. These results provide a reference for proposing highly efficient precision intensive management to optimize the expression of target genes.
杨属植物是一种重要的速生、高产树种，广泛种植于世界中纬度平原地区。然而，集约化栽培管理对人工林基因表达的影响在很大程度上仍未得到探索。
精准水肥一体化管理显著提高了欧美杨‘Neva’人工林氮素转运、同化和光合作用关键酶活性（比 CK 高 1.12-2.63 倍）。同时，这种管理方式对人工林的基因表达具有显著的调控作用。与常规灌溉相比，滴灌（ND）处理下鉴定到 1554 个差异表达基因（DEGs）。与 ND 相比，在三种滴灌施肥组合下，鉴定到 2761-4116 个 DEGs，主要呈上调表达，其中 202 个 DEGs主要受施肥调控。此外，滴灌通过降低细胞壁合成相关基因的表达来减少不必要的水分运输。精准滴灌和水肥一体化管理促进了碳氮代谢的协同调控，上调了氮素转运和同化过程中主要基因的表达（5 个 DEGs）、光合作用（15 个 DEGs）和植物激素信号转导（11 个 DEGs）。微量元素的添加进一步增强了次生代谢过程基因的上调表达。此外，共表达网络鉴定出 9 个受精准水肥一体化管理调控的关键基因，表明它们在调控杨树生长方面发挥着重要作用。
精准水肥一体化管理能够调控与碳氮代谢途径、植物激素信号转导相关的关键基因和转录因子基因的表达，增强相关过程中关键酶的活性。这种调控促进了氮的吸收和利用，以及光捕获、光传输和电子传递等光合作用能力，微弱地协同调节人工林的生长。这些结果为提出高效精准集约管理以优化目标基因的表达提供了参考。
