Moon Jungho, Lee Yunbeom, Ihee Hyotcherl
Center for Advanced Reaction Dynamics (CARD), Institute for Basic Science (IBS), Daejeon 34141, Republic of Korea.
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Chem Commun (Camb). 2024 Aug 29;60(71):9472-9482. doi: 10.1039/d4cc03185g.
Time-resolved serial femtosecond crystallography (TR-SFX) has emerged as a crucial tool for studying the structural dynamics of proteins. In principle, TR-SFX has the potential to be a powerful tool not only for studying proteins but also for investigating chemical reactions. However, non-protein systems generally face challenges in indexing due to sparse Bragg spots and encounter difficulties in effectively exciting target molecules. Nevertheless, successful TR-SFX studies on chemical systems have been recently reported in a few instances, boding well for the application of TR-SFX to study chemical reactions in the future. In this context, we review the static SFX and TR-SFX studies conducted on chemical systems reported to date and suggest prospects for future research directions.
时间分辨飞秒晶体学连续成像（TR-SFX）已成为研究蛋白质结构动力学的关键工具。原则上，TR-SFX不仅有潜力成为研究蛋白质的强大工具，还可用于研究化学反应。然而，非蛋白质系统通常因布拉格斑点稀疏而在指标化方面面临挑战，并且在有效激发目标分子方面存在困难。尽管如此，最近已有一些关于化学系统的TR-SFX成功研究报道，这为未来将TR-SFX应用于研究化学反应带来了良好的兆头。在此背景下，我们回顾了迄今为止报道的对化学系统进行的静态SFX和TR-SFX研究，并对未来的研究方向提出了展望。
