Samadi Fahad Mansoor, Suhail Shaista, Sonam Manjari, Ahmad Mohd Kaleem, Kumar Vijay, Chandra Shaleen, Mohammad Shadab
Department of Oral Pathology and Microbiology, King George Medical University, Lucknow, India.
Department of Oral Pathology and Microbiology, Sardar Patel PG Institute of Dental and Medical Sciences, Lucknow, India.
Iran J Pathol. 2024 Spring;19(2):146-151. doi: 10.30699/IJP.2024.1996330.3081. Epub 2024 Feb 15.
BACKGROUND & OBJECTIVE: Telomeres consist of repetitive G-rich nucleotides located at the end of each chromosome, acting as protein binding sites. The aim of this study was to examine the differences in telomere length in blood, saliva, and tissue samples at various stages of oral precancerous and cancerous lesions.
Samples of blood, tissue, and saliva were collected from patients with oral precancerous and cancerous lesions. DNA extraction was performed. Then, a TRAP assay was conducted to assess and compare the telomere length and telomerase expression.
The levels of telomerase activity (TA) in the DNA samples ranged from 0.19 to 6.91 (2.05+1.37) among oral squamous cell carcinoma (OSCC) patients and from 0.17 to 4.5 (0.28+4.25) among precancerous patients. A significant difference was observed in TA levels between OSCC and precancerous samples (=3.9691, = 0.0000).
Assessing the telomerase activity is crucial for studying the behavior of carcinoma in the clinical setting. The augmented telomerase expression and the length of telomere contribute to OSCC progression. Hence, this study adds a diagnostic tool that can serve as a biomarker for the early detection and prognosis of OSCC.
端粒由位于每条染色体末端的富含鸟嘌呤的重复核苷酸组成，起蛋白质结合位点的作用。本研究旨在检测口腔癌前病变和癌性病变不同阶段血液、唾液及组织样本中端粒长度的差异。
收集口腔癌前病变和癌性病变患者的血液、组织及唾液样本，进行DNA提取，然后进行端粒重复序列扩增法（TRAP）检测，以评估和比较端粒长度及端粒酶表达。
口腔鳞状细胞癌（OSCC）患者DNA样本中端粒酶活性（TA）水平在0.19至6.91（2.05±1.37）之间，癌前病变患者的TA水平在0.17至4.5（0.28±4.25）之间。OSCC与癌前样本的TA水平存在显著差异（F = 3.9691，P = 0.0000）。
评估端粒酶活性对于在临床环境中研究癌症行为至关重要。端粒酶表达增强和端粒长度与OSCC进展有关。因此，本研究增加了一种可作为OSCC早期检测和预后生物标志物的诊断工具。
