Zaer-Anaqz Zahra, Khakvar Reza, Mohammadi Seyed Abolghasem, Bannazadeh Baghi Hossein, Koolivand Davoud
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Phage (New Rochelle). 2024 Jun 21;5(2):99-106. doi: 10.1089/phage.2023.0023. eCollection 2024 Jun.
Bacterial canker, caused by , is a devastating disease of stone fruit trees worldwide. The bacterium has a broad host range and a high capacity for adaptation and dissemination, owing to its high mutation rate and horizontal gene transfer. Traditional control methods based on copper compounds and antibiotics have resulted in the development of resistance in the bacterial population. Thus, alternative approaches are needed, such as phage therapy. This study aimed to characterize the physicochemical and biological properties of novel pv. ()-specific phages isolated from the soils of northwestern Iran. Seventy-five phage isolates were obtained, and their host range was determined against various bacterial pathogens. Five phages exhibiting the highest lytic activity against and a narrow host range were selected for subsequent analysis. The stability of the selected phages was assessed under different conditions such as ultraviolet irradiation, temperature, pH, NaCl concentration, and chloroform exposure. The selected phages demonstrated significant effectiveness , exerting substantial suppression on the population of . This reduction was observed for both individual phages and when the phages were utilized as a mixture. The findings indicate that phages have the potential to be used as biocontrol agents in agriculture.
由[病原菌名称未给出]引起的细菌性溃疡病是全球核果类果树的一种毁灭性病害。由于其高突变率和水平基因转移，该细菌具有广泛的宿主范围以及很强的适应和传播能力。基于铜化合物和抗生素的传统防治方法已导致细菌群体产生抗性。因此，需要诸如噬菌体疗法等替代方法。本研究旨在表征从伊朗西北部土壤中分离出的新型[病原菌名称未给出]pv.[pv.后具体名称未给出]（[具体菌株名称未给出]）特异性噬菌体的物理化学和生物学特性。获得了75株噬菌体分离株，并测定了它们对各种细菌病原体的宿主范围。选择了5株对[病原菌名称未给出]表现出最高裂解活性且宿主范围狭窄的噬菌体进行后续分析。在紫外线照射、温度、pH值、NaCl浓度和氯仿暴露等不同条件下评估所选噬菌体的稳定性。所选噬菌体显示出显著效果，对[病原菌名称未给出]群体具有显著抑制作用。无论是单个噬菌体还是将噬菌体作为混合物使用时，均观察到这种减少。研究结果表明，噬菌体有潜力在农业中用作生物防治剂。
