Jiang Yanyan, Zhou Shanshan, Yuan Zhongying, Hu Xinyu, Li Zhen, Wang Yaxue, Shen Yujuan, Cao Jianping
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Parasite and Vector Biology, National Center for International Research on Tropical Diseases, WHO Collaborating Centre for Tropical Diseases, Shanghai, China.
School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Vet Sci. 2024 Jul 25;11:1426384. doi: 10.3389/fvets.2024.1426384. eCollection 2024.
, an obligatory intracellular fungus, is prevalent among animals and humans. Due to their close interaction with humans and their extensive regional distribution, brown rats () are important pathogen reservoirs. To assess the zoonotic transmission potential of , a molecular investigation was conducted on 817 from four cities in Heilongjiang Province, China.
A total of 817 R. norvegicus were collected from four cities in Heilongjiang Province, China. The genotyping of E. bieneusi was conducted through PCR amplification of the small subunit ribosomal RNA (SSU rRNA)'s internal transcribed spacer (ITS) segments. Phylogenetic and similarity analyses were used to examine zoonotic potential and genetic characteristics of the E. bieneusi-positive specimens.
Among the 817 the total infection rate was 33.3% (272/817). Seventy-five genotypes were identified, including 14 known genotypes D ( = 167), A ( = 15), HLJ-CP1 ( = 12), WR8 ( = 6), EbpC ( = 2), BEB6 ( = 1), CS-4 ( = 1), CHPM1 ( = 1), Henan-II ( = 1), HNH-22 ( = 1), HNH-25 ( = 1), I ( = 1), JLD-XI ( = 1), SDD5 ( = 1), and 61 novel genotypes designated as SHWR1 ( = 10), SYSWR1 ( = 2), and SHWR2 to SHWR17, SYSWR2 to SYSWR36 and QTHWR1 to QTHWR8 ( = 1, each). Moreover, 10 samples exhibited mixed genotype infections, including D + A ( = 3), D + EbpC ( = 1), D + HLJ-CP1 ( = 1), D + SHWR1 ( = 1), D + SHWR16 ( = 1), D + SHWR17 ( = 1), SDD5 + WR8 ( = 1), and CS-4 + SYSWR36 ( = 1). Phylogenetic analysis grouped the genotypes into three main groups: group 1 ( = 67), group 2 ( = 5), and group 9 ( = 3).
The high prevalence and genetic diversity of in Heilongjiang Province's imply that these animals spread the pathogen. The that carries can spread zoonotic disease, making it a serious hazard to the local human population. Therefore, it is imperative to raise awareness about the dangers posed by and implement measures to reduce their population to prevent environmental contamination.
白念珠菌是一种 obligatory 细胞内真菌，在动物和人类中普遍存在。由于褐家鼠与人类密切接触且分布广泛，它们是重要的病原体宿主。为评估白念珠菌的人畜共患病传播潜力，对中国黑龙江省四个城市的817只褐家鼠进行了分子调查。
从中国黑龙江省四个城市共收集了817只褐家鼠。通过对小亚基核糖体RNA（SSU rRNA）的内部转录间隔区（ITS）片段进行PCR扩增，对白念珠菌进行基因分型。采用系统发育和相似性分析来研究白念珠菌阳性标本的人畜共患病潜力和遗传特征。
在817只褐家鼠中，总感染率为33.3%（272/817）。鉴定出75种基因型，包括14种已知基因型D（n = 167）、A（n = 15）、HLJ - CP1（n = 12）、WR8（n = 6）、EbpC（n = 2）、BEB6（n = 1）、CS - 4（n = 1）、CHPM1（n = 1）、河南 - II（n = 1）、HNH - 22（n = 1）、HNH - 25（n = 1）、I（n = 1）、JLD - XI（n = 1）、SDD5（n = 1），以及61种新基因型，分别命名为SHWR1（n = 10）、SYSWR1（n = 2），以及SHWR2至SHWR17、SYSWR2至SYSWR36和QTHWR1至QTHWR8（各n = 1）。此外，10个样本表现出混合基因型感染，包括D + A（n = 3）、D + EbpC（n = 1）、D + HLJ - CP1（n = 1）、D + SHWR1（n = 1）、D + SHWR16（n = 1）、D + SHWR体17（n = 1）、SDD5 + WR8（n = 1），以及CS - 4 + SYSWR36（n = 1）。系统发育分析将基因型分为三个主要组：第1组（n = 67）、第2组（n = 5）和第9组（n = 3）。
黑龙江省褐家鼠中白念珠菌的高流行率和遗传多样性表明这些动物传播病原体。褐家鼠携带的白念珠菌可传播人畜共患病，对当地人群构成严重危害。因此，必须提高对褐家鼠所构成危险的认识，并采取措施减少其数量以防止环境污染。 （注：原文中“an obligatory intracellular fungus”表述有误，推测应为“Enterocytozoon bieneusi”，译文按纠正后理解翻译，但不确定原文是否如此，供你参考。）
