茉莉酸生物合成基因 SmLOX4 和 SmLOX5 参与茄子的耐热性。
The Jasmonic Acid Biosynthetic Genes SmLOX4 and SmLOX5 Are Involved in Heat Tolerance in Eggplant.
机构信息
Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
出版信息
Plant Cell Physiol. 2024 Nov 13;65(10):1705-1716. doi: 10.1093/pcp/pcae088.
High-temperature stress (HTS) affects the growth and production of vegetable crops, including eggplant (Solanum melongena L.). Jasmonic acid (JA) plays key roles in regulating resistance to biotic and abiotic stresses in plants. Nonetheless, reports on the role of JA in heat tolerance in eggplant are rare. Herein, the effects of JA on heat tolerance in eggplant and the functions of the JA biosynthetic genes SmLOX4 and SmLOX5 were analyzed. The results showed that the JA content increased under high-temperature treatment (HTT) and exogenous methyl jasmonate (MeJA) treatment reduced the damage caused by HTT to eggplant. The expression of SmLOX4 and SmLOX5 was induced by HTT and significantly positively correlated with JA biosynthesis. SmLOX4 and SmLOX5 were localized in chloroplasts. The silencing of SmLOX4 and SmLOX5 by virus-induced gene silencing suppressed the heat tolerance of eggplant, whereas the overexpression of SmLOX4 and SmLOX5 enhanced the heat tolerance of Arabidopsis thaliana. JA content and the expression of JA signaling-related genes decreased in the SmLOX4- and SmLOX5-silenced plants but increased in the OE-SmLOX4 and OE-SmLOX5 transgenic plants. These results revealed that SmLOX4 and SmLOX5 improved eggplant heat tolerance by mediating JA biosynthesis and JA signaling pathways.
高温胁迫（HTS）会影响蔬菜作物的生长和产量，包括茄子（Solanum melongena L.）。茉莉酸（JA）在植物应对生物和非生物胁迫的过程中发挥着关键作用。然而，关于 JA 在茄子耐热性中的作用的报道却很少。本研究分析了 JA 对茄子耐热性的影响以及 JA 生物合成基因 SmLOX4 和 SmLOX5 的功能。结果表明，高温处理（HTT）会导致 JA 含量增加，而外源茉莉酸甲酯（MeJA）处理则减轻了 HTT 对茄子造成的损伤。SmLOX4 和 SmLOX5 的表达受到 HTT 的诱导，并且与 JA 生物合成呈显著正相关。SmLOX4 和 SmLOX5 定位于叶绿体中。通过病毒诱导的基因沉默沉默 SmLOX4 和 SmLOX5 会抑制茄子的耐热性，而过表达 SmLOX4 和 SmLOX5 则会增强拟南芥的耐热性。SmLOX4 和 SmLOX5 沉默植株中的 JA 含量和 JA 信号相关基因的表达降低，而 OE-SmLOX4 和 OE-SmLOX5 转基因植株中的 JA 含量和 JA 信号相关基因的表达增加。这些结果表明，SmLOX4 和 SmLOX5 通过介导 JA 生物合成和 JA 信号通路来提高茄子的耐热性。
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